Cyclin B Translation Depends on MTOR Activity After Fertilization in Sea Urchin Embryos

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2016 Mar 11

PMID 26962866

Citations 12

Authors

Heloise Chasse

Odile Mulner-Lorillon

Sandrine Boulben

Virginie Glippa

Julia Morales

Patrick Cormier

Affiliations

Soon will be listed here.

Abstract

The cyclin B/CDK1 complex is a key regulator of mitotic entry. Using PP242, a specific ATP-competitive inhibitor of mTOR kinase, we provide evidence that the mTOR signalling pathway controls cyclin B mRNA translation following fertilization in Sphaerechinus granularis and Paracentrotus lividus. We show that PP242 inhibits the degradation of the cap-dependent translation repressor 4E-BP (eukaryotic initiation factor 4E-Binding Protein). PP242 inhibits global protein synthesis, delays cyclin B accumulation, cyclin B/CDK1 complex activation and consequently entry into the mitotic phase of the cell cycle triggered by fertilization. PP242 inhibits cyclin B mRNA recruitment into active polysomes triggered by fertilization. An amount of cyclin B mRNA present in active polysomes appears to be insensitive to PP242 treatment. Taken together, our results suggest that, following sea urchin egg fertilization, cyclin B mRNA translation is controlled by two independent mechanisms: a PP242-sensitive and an additional PP242-insentitive mechanism.

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