Radiation Damage and Derivatization in Macromolecular Crystallography: a Structure Factor's Perspective

Overview

Journal Acta Crystallogr D Struct Biol

Specialty Biochemistry

Date 2016 Mar 10

PMID 26960125

Citations 6

Authors

Robin L Owen

Darren A Sherrell

Affiliations

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Abstract

During, or even after, data collection the presence and effects of radiation damage in macromolecular crystallography may not always be immediately obvious. Despite this, radiation damage is almost always present, with site-specific damage occurring on very short time (dose) scales well before global damage becomes apparent. A result of both site-specific radiation damage and derivatization is a change in the relative intensity of reflections. The size and approximate rate of onset of X-ray-induced transformations is compared with the changes expected from derivatization, and strategies for minimizing radiation damage are discussed.

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