Article: Tryptophan-induced pathogenesis of breast cancer

Background: The pathogenesis of breast cancer remains unclear.

Aims: To investigate the pathogenesis of breast cancer through targeted metabolomics of amino acids components in serum of patients with breast cancer.

Methods: Patients with breast cancers were enrolled in our hospital between year January 1(st), 2013 and December 31(st), 2014. Targeted analysis of amino acids was performed using ESI-QTOF-MS instrument. In vitro experiment was performed to determine the influence of tryptophan towards interleukin-10 (IL-10) secretion by CD4+ T cell.

Results: Targeted metabolomics of amino acids showed that the level of tryptophan significantly (p<0.05) increased in patients with breast cancer. Furthermore, the biological function of tryptophan was determined through determining the influence of tryptophan towards IL-10 secretion using in vitro method. The addition of tryptophan (100 uM) in the cell medium can significantly inhibited the secretion of IL-10 by CD4+ T cells, as indicated by the mRNA level and protein concentration.

Conclusion: The inhibition of IL-10 secretion by CD4+ T cells is a potential pathogenesis of breast cancer.

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References

1.

Moore K, de Waal Malefyt R, Coffman R, OGarra A . Interleukin-10 and the interleukin-10 receptor. Annu Rev Immunol. 2001; 19:683-765. DOI: 10.1146/annurev.immunol.19.1.683. View

2.

Fang Z, Gonzalez F . LC-MS-based metabolomics: an update. Arch Toxicol. 2014; 88(8):1491-502. PMC: 6310611. DOI: 10.1007/s00204-014-1234-6. View

3.

Hsia T, Chang W, Liang S, Chen W, Tu C, Chen H . Interleukin-10 (IL-10) promoter genotypes are associated with lung cancer risk in Taiwan males and smokers. Anticancer Res. 2014; 34(12):7039-44. View

4.

Chard L, Maniati E, Wang P, Zhang Z, Gao D, Wang J . A vaccinia virus armed with interleukin-10 is a promising therapeutic agent for treatment of murine pancreatic cancer. Clin Cancer Res. 2014; 21(2):405-16. DOI: 10.1158/1078-0432.CCR-14-0464. View

5.

Jenabian M, El-Far M, Vyboh K, Kema I, Costiniuk C, Thomas R . Immunosuppressive Tryptophan Catabolism and Gut Mucosal Dysfunction Following Early HIV Infection. J Infect Dis. 2015; 212(3):355-66. DOI: 10.1093/infdis/jiv037. View

Tryptophan-induced Pathogenesis of Breast Cancer