Selective Expression of Transthyretin in Subtypes of Lung Cancer

Overview

Journal J Mol Histol

Specialty Biochemistry

Date 2016 Mar 5

PMID 26943652

Citations 8

Authors

Shuai Hao

Suozhu Sun

Xueyuan Xiao

Dacheng He

Liyun Liu

Affiliations

Soon will be listed here.

Abstract

Transthyretin (TTR) is expressed primarily in liver, choroid plexus of brain and pancreatic islet A and B cells. It is also synthesized in some endocrine tumors. In the present study, the protein expression of TTR in lung cancer tissues and cell lines was investigated by western blot. The mRNA expression of TTR in 24 pairs of frozen lung cancer tissues was examined by RT-PCR. The specific expression and cellular distribution of TTR were also evaluated in 104 paraffin-embedded lung cancer samples and 3 normal lung tissues by immunohistochemistry. Similarly, the subcellular localization and expression of TTR were further analyzed in lung cancer cell lines. With the exception of mucinous adenocarcinoma, the expression of TTR protein was observed in all tested subtypes of lung carcinoma. Adenocarcinoma displayed the highest positive expression rate of TTR, accounting for 84.4 %, and the positive expression rate of TTR was up to 85.7 % at stages III and IV. The secretory bubbles with strong TTR staining were observed in luminal cells of lung cancer. Furthermore, the localization of TTR in the cytoplasm of lung cancer cells and the secretion of TTR into extracellular milieu were also confirmed. Taken together, TTR is selectively synthesized in lung cancer cells and can be secreted extracellularly.

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References

Jacobsson B, Collins V, Grimelius L, Pettersson T, Sandstedt B, Carlstrom A . Transthyretin immunoreactivity in human and porcine liver, choroid plexus, and pancreatic islets. J Histochem Cytochem. 1989; 37(1):31-7. DOI: 10.1177/37.1.2642294. View

Guo D, Hu K, Lei Y, Wang Y, Ma T, He D . Identification and characterization of a novel cytoplasm protein ICF45 that is involved in cell cycle regulation. J Biol Chem. 2004; 279(51):53498-505. DOI: 10.1074/jbc.M406737200. View

Chen J, Chen L, Xia Y, Zhou H, Yang R, Wu W . Identification and verification of transthyretin as a potential biomarker for pancreatic ductal adenocarcinoma. J Cancer Res Clin Oncol. 2013; 139(7):1117-27. DOI: 10.1007/s00432-013-1422-4. View

Geho D, Liotta L, Petricoin E, Zhao W, Araujo R . The amplified peptidome: the new treasure chest of candidate biomarkers. Curr Opin Chem Biol. 2006; 10(1):50-5. DOI: 10.1016/j.cbpa.2006.01.008. View

Ang L, Debowski T, Michalski R . Immunolocalization of prealbumin (transthyretin) in renal cell carcinoma. Histopathology. 1991; 18(6):565-8. DOI: 10.1111/j.1365-2559.1991.tb01487.x. View

Fung E, Yip T, Lomas L, Wang Z, Yip C, Meng X . Classification of cancer types by measuring variants of host response proteins using SELDI serum assays. Int J Cancer. 2005; 115(5):783-9. DOI: 10.1002/ijc.20928. View

Itoh N, Hanafusa T, Miyagawa J, Tamura S, Inada M, Kawata S . Transthyretin (prealbumin) in the pancreas and sera of newly diagnosed type I (insulin-dependent) diabetic patients. J Clin Endocrinol Metab. 1992; 74(6):1372-7. DOI: 10.1210/jcem.74.6.1592883. View

Wang J, Wei H, Zhao B, Li M, Lv W, Lv L . The reverse effect of X-ray irradiation on acquired gefitinib resistance in non-small cell lung cancer cell line NCI-H1975 in vitro. J Mol Histol. 2014; 45(6):641-52. DOI: 10.1007/s10735-014-9583-2. View

Suresh U, Wilkes S, Hasleton P . Prealbumin in the diagnosis of bronchopulmonary carcinoid tumours. J Clin Pathol. 1991; 44(7):573-5. PMC: 496797. DOI: 10.1136/jcp.44.7.573. View

10.

Menard C, Johann D, Lowenthal M, Muanza T, Sproull M, Ross S . Discovering clinical biomarkers of ionizing radiation exposure with serum proteomic analysis. Cancer Res. 2006; 66(3):1844-50. DOI: 10.1158/0008-5472.CAN-05-3466. View

11.

Marten N, Sladek F, Straus D . Effect of dietary protein restriction on liver transcription factors. Biochem J. 1996; 317 ( Pt 2):361-70. PMC: 1217496. DOI: 10.1042/bj3170361. View

12.

Li M, Ye B, Zhang Y, Chen H, Xia D, Liu M . Proteomic analysis of serum in lung cancer induced by 3-methylcholanthrene. J Biomed Biotechnol. 2009; 2009:397910. PMC: 2753780. DOI: 10.1155/2009/397910. View

13.

Ni T, Mao G, Xue Q, Liu Y, Chen B, Cui X . Upregulated expression of ILF2 in non-small cell lung cancer is associated with tumor cell proliferation and poor prognosis. J Mol Histol. 2015; 46(4-5):325-35. DOI: 10.1007/s10735-015-9624-5. View

14.

Ye Y, Liu S, Wu C, Sun Z . TGFβ modulates inflammatory cytokines and growth factors to create premetastatic microenvironment and stimulate lung metastasis. J Mol Histol. 2015; 46(4-5):365-75. DOI: 10.1007/s10735-015-9633-4. View

15.

Kozak K, Su F, Whitelegge J, Faull K, Reddy S, Farias-Eisner R . Characterization of serum biomarkers for detection of early stage ovarian cancer. Proteomics. 2005; 5(17):4589-96. DOI: 10.1002/pmic.200500093. View

16.

Imanishi T . Clinical and experimental studies on the profiles of serum proteins in acute hepatic injury. Gastroenterol Jpn. 1981; 16(5):493-505. DOI: 10.1007/BF02774521. View

17.

Youlden D, Cramb S, Baade P . The International Epidemiology of Lung Cancer: geographical distribution and secular trends. J Thorac Oncol. 2008; 3(8):819-31. DOI: 10.1097/JTO.0b013e31818020eb. View

18.

Maciel C, Junqueira M, Paschoal M, Kawamura M, Duarte R, Carvalho M . Differential proteomic serum pattern of low molecular weight proteins expressed by adenocarcinoma lung cancer patients. J Exp Ther Oncol. 2006; 5(1):31-8. View

19.

Raghu P, Sivakumar B . Interactions amongst plasma retinol-binding protein, transthyretin and their ligands: implications in vitamin A homeostasis and transthyretin amyloidosis. Biochim Biophys Acta. 2004; 1703(1):1-9. DOI: 10.1016/j.bbapap.2004.09.023. View

20.

Chatterji B, Borlak J . Serum proteomics of lung adenocarcinomas induced by targeted overexpression of c-raf in alveolar epithelium identifies candidate biomarkers. Proteomics. 2007; 7(21):3980-91. DOI: 10.1002/pmic.200700290. View