Activity of Medicinal Plant Extracts on Multiplication of Mycobacterium Tuberculosis Under Reduced Oxygen Conditions Using Intracellular and Axenic Assays

Overview

Journal Int J Microbiol

Publisher Wiley

Specialty Microbiology

Date 2016 Mar 5

PMID 26941797

Citations 1

Authors

Purva D Bhatter

Pooja D Gupta

Tannaz J Birdi

Affiliations

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Abstract

Aim. Test the activity of selected medicinal plant extracts on multiplication of Mycobacterium tuberculosis under reduced oxygen concentration which represents nonreplicating conditions. Material and Methods. Acetone, ethanol and aqueous extracts of the plants Acorus calamus L. (rhizome), Ocimum sanctum L. (leaf), Piper nigrum L. (seed), and Pueraria tuberosa DC. (tuber) were tested on Mycobacterium tuberculosis H37Rv intracellularly using an epithelial cell (A549) infection model. The extracts found to be active intracellularly were further studied axenically under reducing oxygen concentrations. Results and Conclusions. Intracellular multiplication was inhibited ≥60% by five of the twelve extracts. Amongst these 5 extracts, in axenic culture, P. nigrum (acetone) was active under aerobic, microaerophilic, and anaerobic conditions indicating presence of multiple components acting at different levels and P. tuberosa (aqueous) showed bactericidal activity under microaerophilic and anaerobic conditions implying the influence of anaerobiosis on its efficacy. P. nigrum (aqueous) and A. calamus (aqueous and ethanol) extracts were not active under axenic conditions but only inhibited intracellular growth of Mycobacterium tuberculosis, suggesting activation of host defense mechanisms to mediate bacterial killing rather than direct bactericidal activity.

Citing Articles

Phytochemical and Antimicrobial Characterization.

de Souza Pereira J, Pereira A, Jandu J, da Paz J, Crovella S, Dos Santos Correia M Front Microbiol. 2017; 8:52.

PMID: 28174564 PMC: 5258698. DOI: 10.3389/fmicb.2017.00052.

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