Evaluation of MRNA Expression of the Transcription Factors of Th1 and Th2 Subsets (T-bet and GATA-3) in Periodontal Health and Disease - A Pilot Study in South Indian Population

Overview

Journal J Indian Soc Periodontol

Date 2016 Mar 5

PMID 26941511

Citations 2

Authors

Nichenametla Rajesh

Kurumathur Vasudevan Arun

Tirumelveli Saravanan Subbu Kumar

Kondareddy Krishna Mohan Reddy

Swarna Alamelu

Bhimavarapu Ravinder Reddy

Affiliations

Soon will be listed here.

Abstract

Background: Based on their respective pro- or anti-inflammatory cytokine profiles, the Th1/Th2 paradigm explains pathogenic mechanisms involved in periodontal disease. Establishment of Th1 and Th2 subsets from a naive T-cell precursor depends on transcriptional regulation. The aim of this study was to compare the expression of master transcription factor regulators T-bet and GATA-3, respectively, to indicate the predominance of Th1 and Th2 subsets in the presence and absence of periodontal disease.

Materials And Methods: A gingival tissue biopsy sample was obtained from each of 10 severe periodontitis patients (>5 mm attachment loss) and 10 periodontally healthy patients (no attachment loss). Biopsies were immediately processed by real-time reverse transcriptase polymerase chain reaction and the difference in mRNA expression of T-bet and GATA-3 was assessed for each group.

Results: The mRNA expression of T-bet was marginally increased about 1.31-fold in disease, while the GATA-3 levels showed a significant decrease of 4.39-fold in disease.

Conclusion: The advanced periodontal lesions lack Th2 cells, which produce anti-inflammatory cytokines. The biopsies were therefore dominated by Th1 cells, which activate macrophages and osteoclasts.

Citing Articles

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