Are PECTIN ESTERASE INHIBITOR Genes Involved in Mediating Resistance to Rhynchosporium Commune in Barley?

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2016 Mar 4

PMID 26937960

Citations 11

Authors

Stephan Marzin

Anja Hanemann

Shailendra Sharma

Gotz Hensel

Jochen Kumlehn

Gunther Schweizer

Marion S Roder

Affiliations

Soon will be listed here.

Abstract

A family of putative PECTIN ESTERASE INHIBITOR (PEI) genes, which were detected in the genomic region co-segregating with the resistance gene Rrs2 against scald caused by Rhynchosporium commune in barley, were characterized and tested for their possible involvement in mediating resistance to the pathogen by complementation and overexpression analysis. The sequences of the respective genes were derived from two BAC contigs originating from the susceptible cultivar 'Morex'. For the genes HvPEI2, HvPEI3, HvPEI4 and HvPEI6, specific haplotypes for 18 resistant and 23 susceptible cultivars were detected after PCR-amplification and haplotype-specific CAPS-markers were developed. None of the tested candidate genes HvPEI2, HvPEI3 and HvPEI4 alone conferred a high resistance level in transgenic over-expression plants, though an improvement of the resistance level was observed especially with OE-lines for gene HvPEI4. These results do not confirm but also do not exclude an involvement of the PEI gene family in the response to the pathogen. A candidate for the resistance gene Rrs2 could not be identified yet. It is possible that Rrs2 is a PEI gene or another type of gene which has not been detected in the susceptible cultivar 'Morex' or the full resistance reaction requires the presence of several PEI genes.

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References

Zaffarano P, McDonald B, Zala M, Linde C . Global Hierarchical Gene Diversity Analysis Suggests the Fertile Crescent Is Not the Center of Origin of the Barley Scald Pathogen Rhynchosporium secalis. Phytopathology. 2008; 96(9):941-50. DOI: 10.1094/PHYTO-96-0941. View

Hensel G, Kastner C, Oleszczuk S, Riechen J, Kumlehn J . Agrobacterium-mediated gene transfer to cereal crop plants: current protocols for barley, wheat, triticale, and maize. Int J Plant Genomics. 2009; 2009:835608. PMC: 2699555. DOI: 10.1155/2009/835608. View

Hensel G, Valkov V, Middlefell-Williams J, Kumlehn J . Efficient generation of transgenic barley: the way forward to modulate plant-microbe interactions. J Plant Physiol. 2007; 165(1):71-82. DOI: 10.1016/j.jplph.2007.06.015. View

Hofmann K, Silvar C, Casas A, Herz M, Buttner B, Gracia M . Fine mapping of the Rrs1 resistance locus against scald in two large populations derived from Spanish barley landraces. Theor Appl Genet. 2013; 126(12):3091-102. DOI: 10.1007/s00122-013-2196-4. View

Chen M, Citovsky V . Systemic movement of a tobamovirus requires host cell pectin methylesterase. Plant J. 2003; 35(3):386-92. DOI: 10.1046/j.1365-313x.2003.01818.x. View

Lionetti V, Raiola A, Cervone F, Bellincampi D . Transgenic expression of pectin methylesterase inhibitors limits tobamovirus spread in tobacco and Arabidopsis. Mol Plant Pathol. 2013; 15(3):265-74. PMC: 6638747. DOI: 10.1111/mpp.12090. View

Ariyadasa R, Mascher M, Nussbaumer T, Schulte D, Frenkel Z, Poursarebani N . A sequence-ready physical map of barley anchored genetically by two million single-nucleotide polymorphisms. Plant Physiol. 2013; 164(1):412-23. PMC: 3875818. DOI: 10.1104/pp.113.228213. View

Di Matteo A, Giovane A, Raiola A, Camardella L, Bonivento D, De Lorenzo G . Structural basis for the interaction between pectin methylesterase and a specific inhibitor protein. Plant Cell. 2005; 17(3):849-58. PMC: 1069703. DOI: 10.1105/tpc.104.028886. View

Juge N . Plant protein inhibitors of cell wall degrading enzymes. Trends Plant Sci. 2006; 11(7):359-67. DOI: 10.1016/j.tplants.2006.05.006. View

10.

Osorio S, Castillejo C, Quesada M, Medina-Escobar N, Brownsey G, Suau R . Partial demethylation of oligogalacturonides by pectin methyl esterase 1 is required for eliciting defence responses in wild strawberry (Fragaria vesca). Plant J. 2007; 54(1):43-55. DOI: 10.1111/j.1365-313X.2007.03398.x. View

11.

Bjornstad A, Patil V, Tekauz A, Maroy A, Skinnes H, Jensen A . Resistance to Scald (Rhynchosporium secalis) in Barley (Hordeum vulgare) Studied by Near-Isogenic Lines: I. Markers and Differential Isolates. Phytopathology. 2008; 92(7):710-20. DOI: 10.1094/PHYTO.2002.92.7.710. View

12.

Avrova A, Knogge W . Rhynchosporium commune: a persistent threat to barley cultivation. Mol Plant Pathol. 2012; 13(9):986-97. PMC: 6638709. DOI: 10.1111/j.1364-3703.2012.00811.x. View

13.

Hothorn M, Wolf S, Aloy P, Greiner S, Scheffzek K . Structural insights into the target specificity of plant invertase and pectin methylesterase inhibitory proteins. Plant Cell. 2004; 16(12):3437-47. PMC: 535884. DOI: 10.1105/tpc.104.025684. View

14.

Sakata K, Nagamura Y, Numa H, Antonio B, Nagasaki H, Idonuma A . RiceGAAS: an automated annotation system and database for rice genome sequence. Nucleic Acids Res. 2001; 30(1):98-102. PMC: 99141. DOI: 10.1093/nar/30.1.98. View

15.

Hanemann A, Schweizer G, Cossu R, Wicker T, Roder M . Fine mapping, physical mapping and development of diagnostic markers for the Rrs2 scald resistance gene in barley. Theor Appl Genet. 2009; 119(8):1507-22. DOI: 10.1007/s00122-009-1152-9. View

16.

Cantu D, Vicente A, Labavitch J, Bennett A, Powell A . Strangers in the matrix: plant cell walls and pathogen susceptibility. Trends Plant Sci. 2008; 13(11):610-7. DOI: 10.1016/j.tplants.2008.09.002. View

17.

Yang L, Ding J, Zhang C, Jia J, Weng H, Liu W . Estimating the copy number of transgenes in transformed rice by real-time quantitative PCR. Plant Cell Rep. 2004; 23(10-11):759-63. DOI: 10.1007/s00299-004-0881-0. View

18.

Himmelbach A, Liu L, Zierold U, Altschmied L, Maucher H, Beier F . Promoters of the barley germin-like GER4 gene cluster enable strong transgene expression in response to pathogen attack. Plant Cell. 2010; 22(3):937-52. PMC: 2861458. DOI: 10.1105/tpc.109.067934. View

19.

Giovane A, Servillo L, Balestrieri C, Raiola A, DAvino R, Tamburrini M . Pectin methylesterase inhibitor. Biochim Biophys Acta. 2004; 1696(2):245-52. DOI: 10.1016/j.bbapap.2003.08.011. View

20.

Lionetti V, Cervone F, Bellincampi D . Methyl esterification of pectin plays a role during plant-pathogen interactions and affects plant resistance to diseases. J Plant Physiol. 2012; 169(16):1623-30. DOI: 10.1016/j.jplph.2012.05.006. View