Curing of the Killer Character of Saccharomyces Cerevisiae with Acridine Orange

Overview

Journal FEMS Microbiol Lett

Publisher Oxford University Press

Specialty Microbiology

Date 1989 Nov 1

PMID 2693197

Citations 4

Authors

J Cansado

E Longo

D Agrelo

T G Villa

Affiliations

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Abstract

Acridine orange, an intercalating dye usually employed in the curing of bacterial plasmids, was tested for its ability to cure K1 and K2 killer strains (laboratory and wine strains). The results showed a high curing percentage of the killer character. This was demonstrated by the loss of M1 or M2 dsRNAs (responsible for toxin production and resistance to it) and because the meiotic products exhibited non-Mendelian segregation. The curing percentages varied, depending on the strain but not on the killer type, and showed similar efficiency as compared with other known curing agents.

Citing Articles

Influence of the curing of the killer phenotype inSaccharomyces cerevisiae wine strains on their fermentative behaviour.

Longo E, Cansado J, Sieiro C, Calo P, Velazquez J, Villa T World J Microbiol Biotechnol. 2014; 8(2):147-50.

PMID: 24425397 DOI: 10.1007/BF01195835.

Monitoring of killer yeast populations in mixed cultures: influence of incubation temperature of microvinifications samples.

Maturano Y, Nally M, Toro M, de Figueroa L, Combina M, Vazquez F World J Microbiol Biotechnol. 2012; 28(11):3135-42.

PMID: 22806751 DOI: 10.1007/s11274-012-1123-1.

Yeast killer systems.

Magliani W, Conti S, Gerloni M, Bertolotti D, Polonelli L Clin Microbiol Rev. 1997; 10(3):369-400.

PMID: 9227858 PMC: 172926. DOI: 10.1128/CMR.10.3.369.

Isolation of a dsRNA virus from Dipodascus (Endomyces) magnusii.

Nosek J, Filipp D, Bederkova K, Griac P Curr Genet. 1993; 23(3):219-22.

PMID: 8435850 DOI: 10.1007/BF00351499.