Polyphasic Approach Including MALDI-TOF MS/MS Analysis for Identification and Characterisation of Fusarium Verticillioides in Brazilian Corn Kernels

Overview

Journal Toxins (Basel)

Publisher MDPI

Specialty Toxicology

Date 2016 Mar 2

PMID 26927172

Citations 6

Authors

Susane Chang

Mariele Porto Carneiro-Leao

Benny Ferreira de Oliveira

Cristina Souza-Motta

Nelson Lima

Cledir Santos

Neiva Tinti de Oliveira

Affiliations

Soon will be listed here.

Abstract

Fusarium verticillioides is considered one of the most important global sources of fumonisins contamination in food and feed. Corn is one of the main commodities produced in the Northeastern Region of Brazil. The present study investigated potential mycotoxigenic fungal strains belonging to the F. verticillioides species isolated from corn kernels in 3 different Regions of the Brazilian State of Pernambuco. A polyphasic approach including classical taxonomy, molecular biology, MALDI-TOF MS and MALDI-TOF MS/MS for the identification and characterisation of the F. verticillioides strains was used. Sixty F. verticillioides strains were isolated and successfully identified by classical morphology, proteomic profiles of MALDI-TOF MS, and by molecular biology using the species-specific primers VERT-1 and VERT-2. FUM1 gene was further detected for all the 60 F. verticillioides by using the primers VERTF-1 and VERTF-2 and through the amplification profiles of the ISSR regions using the primers (GTG)₅ and (GACA)₄. Results obtained from molecular analysis shown a low genetic variability among these isolates from the different geographical regions. All of the 60 F. verticillioides isolates assessed by MALDI-TOF MS/MS presented ion peaks with the molecular mass of the fumonisin B1 (721.83 g/mol) and B2 (705.83 g/mol).

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