A Highly Sensitive Monoclonal Antibody Based Biosensor for Quantifying 3-5 Ring Polycyclic Aromatic Hydrocarbons (PAHs) in Aqueous Environmental Samples

Overview

Journal Sens Biosensing Res

Specialty Biotechnology

Date 2016 Mar 1

PMID 26925369

Citations 13

Authors

Xin Li

Stephen L Kaattari

Mary A Vogelbein

George G Vadas

Michael A Unger

Affiliations

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Abstract

Immunoassays based on monoclonal antibodies (mAbs) are highly sensitive for the detection of polycyclic aromatic hydrocarbons (PAHs) and can be employed to determine concentrations in near real-time. A sensitive generic mAb against PAHs, named as 2G8, was developed by a three-step screening procedure. It exhibited nearly uniformly high sensitivity against 3-ring to 5-ring unsubstituted PAHs and their common environmental methylated PAHs, with IC values between 1.68-31 μg/L (ppb). 2G8 has been successfully applied on the KinExA Inline Biosensor system for quantifying 3-5 ring PAHs in aqueous environmental samples. PAHs were detected at a concentration as low as 0.2 μg/L. Furthermore, the analyses only required 10 min for each sample. To evaluate the accuracy of the 2G8-based biosensor, the total PAH concentrations in a series of environmental samples analyzed by biosensor and GC-MS were compared. In most cases, the results yielded a good correlation between methods. This indicates that generic antibody 2G8 based biosensor possesses significant promise for a low cost, rapid method for PAH determination in aqueous samples.

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