Dynamics of Snake-like Swarming Behavior of Vibrio Alginolyticus

Overview

Journal Biophys J

Publisher Cell Press

Specialty Biophysics

Date 2016 Feb 25

PMID 26910435

Citations 9

Authors

Thomas Bottcher

Hunter L Elliott

Jon Clardy

Affiliations

Soon will be listed here.

Abstract

Swarming represents a special case of bacterial behavior where motile bacteria migrate rapidly and collectively on surfaces. Swarming and swimming motility of bacteria has been studied well for rigid, self-propelled rods. In this study we report a strain of Vibrio alginolyticus, a species that exhibits similar collective motility but a fundamentally different cell morphology with highly flexible snake-like swarming cells. Investigating swarming dynamics requires high-resolution imaging of single cells with coverage over a large area: thousands of square microns. Researchers previously have employed various methods of motion analysis but largely for rod-like bacteria. We employ temporal variance analysis of a short time-lapse microscopic image series to capture the motion dynamics of swarming Vibrio alginolyticus at cellular resolution over hundreds of microns. Temporal variance is a simple and broadly applicable method for analyzing bacterial swarming behavior in two and three dimensions with both high-resolution and wide-spatial coverage. This study provides detailed insights into the swarming architecture and dynamics of Vibrio alginolyticus isolate B522 on carrageenan agar that may lay the foundation for swarming studies of snake-like, nonrod-shaped motile cell types.

Citing Articles

Surveying a Swarm: Experimental Techniques To Establish and Examine Bacterial Collective Motion.

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Use of Alternative Gelling Agents Reveals the Role of Rhamnolipids in Surface Motility.

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Pattern formation and polarity sorting of driven actin filaments on lipid membranes.

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The release of a distinct cell type from swarm colonies facilitates dissemination of Vibrio parahaemolyticus in the environment.

Freitas C, Glatter T, Ringgaard S ISME J. 2019; 14(1):230-244.

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