PSMB8 and PBK As Potential Gastric Cancer Subtype-specific Biomarkers Associated with Prognosis

Overview

Journal Oncotarget

Specialty Oncology

Date 2016 Feb 20

PMID 26894977

Citations 41

Authors

Chae Hwa Kwon

Hye Ji Park

Yu Ri Choi

Ahrong Kim

Hye Won Kim

Jin Hwa Choi

Chung Su Hwang

So Jung Lee

Chang In Choi

Tae Yong Jeon

Dae Hwan Kim

Gwang Ha Kim

Do Youn Park

Affiliations

Soon will be listed here.

Abstract

Gastric adenocarcinoma is a common form of cancer associated with a poor prognosis. We analyzed microarray profiling data from 48 patients with gastric adenocarcinoma to characterize gastric cancer subtypes and identify biomarkers associated with prognosis. We identified two major subtypes of gastric adenocarcinoma differentially associated with overall survival (P = 0.025). Genes that were differentially expressed were identified using specific criteria (P < 0.001 and >1.5-fold); expression of 294 and 116 genes was enriched in good and poor prognosis subtypes, respectively. Genes related to translational elongation and cell cycle were upregulated in the poor prognosis group. Of these genes, upregulation of proteasome subunit beta type 8 PSMB8 and PDZ binding kinase PBK was confirmed by real-time reverse transcription-PCR analysis. PSMB8 or PBK knockdown had no effect on gastric cancer cell proliferation but suppressed cell migration and invasion, respectively. Furthermore, immunohistochemistry analysis of 385 gastric cancer patients revealed that increased nuclear expression of PSMB8 and PBK was correlated with depth of invasion, lymph node metastasis, and lower survival rates. Taken together, two gastric adenocarcinoma subtypes were predictive of prognosis. PSMB8 and PBK were predictive of gastric cancer prognosis and could be potential gastric cancer subtype-specific biomarkers.

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