Hyperspectral Imaging for Burn Depth Assessment in an Animal Model

Overview

Journal Plast Reconstr Surg Glob Open

Specialty General Surgery

Date 2016 Feb 20

PMID 26894016

Citations 12

Authors

Michael S Chin

Oksana Babchenko

Jorge Lujan-Hernandez

Lisa Nobel

Ronald Ignotz

Janice F Lalikos

Affiliations

Soon will be listed here.

Abstract

Unlabelled: Differentiating between superficial and deep-dermal (DD) burns remains challenging. Superficial-dermal burns heal with conservative treatment; DD burns often require excision and skin grafting. Decision of surgical treatment is often delayed until burn depth is definitively identified. This study's aim is to assess the ability of hyperspectral imaging (HSI) to differentiate burn depth.

Methods: Thermal injury of graded severity was generated on the dorsum of hairless mice with a heated brass rod. Perfusion and oxygenation parameters of injured skin were measured with HSI, a noninvasive method of diffuse reflectance spectroscopy, at 2 minutes, 1, 24, 48 and 72 hours after wounding. Burn depth was measured histologically in 12 mice from each burn group (n = 72) at 72 hours.

Results: Three levels of burn depth were verified histologically: intermediate-dermal (ID), DD, and full-thickness. At 24 hours post injury, total hemoglobin (tHb) increased by 67% and 16% in ID and DD burns, respectively. In contrast, tHb decreased to 36% of its original levels in full-thickness burns. Differences in deoxygenated and tHb among all groups were significant (P < 0.001) at 24 hours post injury.

Conclusions: HSI was able to differentiate among 3 discrete levels of burn injury. This is likely because of its correlation with skin perfusion: superficial burn injury causes an inflammatory response and increased perfusion to the burn site, whereas deeper burns destroy the dermal microvasculature and a decrease in perfusion follows. This study supports further investigation of HSI in early burn depth assessment.

Citing Articles

visualization of burn depth in skin tissue of rats using hemoglobin parameters estimated by diffuse reflectance spectral imaging.

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Non-invasive medical imaging technology for the diagnosis of burn depth.

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Noninvasive hemoglobin sensing and imaging: optical tools for disease diagnosis.

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Surgical spectral imaging.

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Imaging in Chronic Wound Diagnostics.

Li S, Mohamedi A, Senkowsky J, Nair A, Tang L Adv Wound Care (New Rochelle). 2020; 9(5):245-263.

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