Proteome Analysis of Disease Resistance Against Ralstonia Solanacearum in Potato Cultivar CT206-10

Overview

Journal Plant Pathol J

Date 2016 Feb 19

PMID 26889112

Citations 8

Authors

Sangryeol Park

Ravi Gupta

R Krishna

Sun Tae Kim

Dong Yeol Lee

Duk-Ju Hwang

Shin-Chul Bae

Il-Pyung Ahn

Affiliations

Soon will be listed here.

Abstract

Potato is one of the most important crops worldwide. Its commercial cultivars are highly susceptible to many fungal and bacterial diseases. Among these, bacterial wilt caused by Ralstonia solanacearum causes significant yield loss. In the present study, integrated proteomics and genomics approaches were used in order to identify bacterial wilt resistant genes from Rs resistance potato cultivar CT-206-10. 2-DE and MALDI-TOF/TOF-MS analysis identified eight differentially abundant proteins including glycine-rich RNA binding protein (GRP), tomato stress induced-1 (TSI-1) protein, pathogenesis-related (STH-2) protein and pentatricopeptide repeat containing (PPR) protein in response to Rs infection. Further, semi-quantitative RT-PCR identified up-regulation in transcript levels of all these genes upon Rs infection. Taken together, our results showed the involvement of the identified proteins in the Rs stress tolerance in potato. In the future, it would be interesting to raise the transgenic plants to further validate their involvement in resistance against Rs in potato.

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