MiRNA-128 Regulates the Proliferation and Neurogenesis of Neural Precursors by Targeting PCM1 in the Developing Cortex

Overview

Journal Elife

Specialty Biology

Date 2016 Feb 18

PMID 26883496

Citations 41

Authors

Wei Zhang

Paul Jong Kim

Zhongcan Chen

Hidayat Lokman

Lifeng Qiu

Ke Zhang

Steven George Rozen

Eng King Tan

Hyunsoo Shawn Je

Li Zeng

Affiliations

Soon will be listed here.

Abstract

During the development, tight regulation of the expansion of neural progenitor cells (NPCs) and their differentiation into neurons is crucial for normal cortical formation and function. In this study, we demonstrate that microRNA (miR)-128 regulates the proliferation and differentiation of NPCs by repressing pericentriolar material 1 (PCM1). Specifically, overexpression of miR-128 reduced NPC proliferation but promoted NPC differentiation into neurons both in vivo and in vitro. In contrast, the reduction of endogenous miR-128 elicited the opposite effects. Overexpression of miR-128 suppressed the translation of PCM1, and knockdown of endogenous PCM1 phenocopied the observed effects of miR-128 overexpression. Furthermore, concomitant overexpression of PCM1 and miR-128 in NPCs rescued the phenotype associated with miR-128 overexpression, enhancing neurogenesis but inhibiting proliferation, both in vitro and in utero. Taken together, these results demonstrate a novel mechanism by which miR-128 regulates the proliferation and differentiation of NPCs in the developing neocortex.

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