Toxigenic Profile of Methicillin-sensitive and Resistant Staphylococcus Aureus Isolated from Special Groups

Overview

Journal Ann Clin Microbiol Antimicrob

Publisher Biomed Central

Specialties Infectious Diseases
Microbiology

Date 2016 Feb 17

PMID 26880287

Citations 2

Authors

Camila Sena Martins de Souza

Carlos Magno Castelo Branco Fortaleza

Claudia Lima Witzel

Monica Silveira

Mariana Favero Bonesso

Silvio Alencar Marques

Maria de Lourdes Ribeiro de Souza da Cunha

Affiliations

Soon will be listed here.

Abstract

Background: Staphylococcus aureus is characterized by its pathogenicity and high prevalence, causing disease in both healthy and immunocompromised individuals due to its easy dissemination. This fact is aggravated by the widespread dissemination of S. aureus carrying toxigenic genes. The objective of this study was to determine the toxigenic profile of methicillin-sensitive S. aureus (MSSA) and methicillin-resistant S. aureus (MRSA) in patients with purulent skin and/or soft tissue infections seen at the Dermatology Department of the University Hospital of the Botucatu Medical School, asymptomatic adults older than 60 years living in nursing homes, and prison inmates of the Avaré Detention Center.

Methods: PCR was used for the detection of the mecA gene, enterotoxin genes (sea, seb, and sec), exfoliative toxins A and B (eta and etb), toxic shock syndrome toxin 1 (tst), panton-valentine leukocidin (lukS-PV and lukF-PV), and alpha- and delta-hemolysins or cytotoxins (hla and hld).

Results: The results showed a significant prevalence of toxigenic genes among S. aureus isolates from asymptomatic individuals, with the observation of a higher prevalence of cytotoxin genes. However, the panton-valentine leukocidin gene was only detected in MSSA isolated from patients with skin infections and the tst gene was exclusively found in MSSA isolated from prison inmates.

Conclusions: The present study demonstrated a significant prevalence of toxigenic genes in MSSA and MRSA strains isolated from asymptomatic S. aureus carriers. There was a higher prevalence of cytotoxin genes.

Citing Articles

Virulence Factors Found in Nasal Colonization and Infection of Methicillin-Resistant (MRSA) Isolates and Their Ability to Form a Biofilm.

Machado T, Pinheiro F, Andre L, Pereira R, Correa R, de Mello G Toxins (Basel). 2020; 13(1).

PMID: 33375552 PMC: 7823648. DOI: 10.3390/toxins13010014.

and Pathogenicity and Polymicrobial Interactions: Lessons beyond Koch's Postulates.

Todd O, Peters B J Fungi (Basel). 2019; 5(3).

PMID: 31487793 PMC: 6787713. DOI: 10.3390/jof5030081.

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