Concentration of Sindbis Virus with Optimized Gradient Insulator-based Dielectrophoresis

Overview

Journal Analyst

Publisher Royal Society of Chemistry

Specialty Chemistry

Date 2016 Feb 16

PMID 26878279

Citations 26

Authors

Jie Ding

Robert M Lawrence

Paul V Jones

Brenda G Hogue

Mark A Hayes

Affiliations

Soon will be listed here.

Abstract

Biotechnology, separation science, and clinical research are impacted by microfluidic devices. Separation and manipulation of bioparticles such as DNA, protein and viruses are performed on these platforms. Microfluidic systems provide many attractive features, including small sample size, rapid detection, high sensitivity and short processing time. Dielectrophoresis (DEP) and electrophoresis are especially well suited to microscale bioparticle control and have been demonstrated in many formats. In this work, an optimized gradient insulator-based DEP device was utilized for concentration of Sindbis virus, an animal virus with a diameter of 68 nm. Within only a few seconds, the concentration of Sindbis virus can be increased by two to six times in the channel under easily accessible voltages as low as about 70 V. Compared with traditional diagnostic methods used in virology, DEP-based microfluidics can enable faster isolation, detection and concentration of viruses in a single step within a short time.

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References

Morgan H, Hughes M, Green N . Separation of submicron bioparticles by dielectrophoresis. Biophys J. 1999; 77(1):516-25. PMC: 1300348. DOI: 10.1016/S0006-3495(99)76908-0. View

Hughes M, Morgan H, Rixon F . Dielectrophoretic manipulation and characterization of herpes simplex virus-1 capsids. Eur Biophys J. 2001; 30(4):268-72. DOI: 10.1007/s002490100144. View

Mackay I, Arden K, Nitsche A . Real-time PCR in virology. Nucleic Acids Res. 2002; 30(6):1292-305. PMC: 101343. DOI: 10.1093/nar/30.6.1292. View

Hughes M, Morgan H, Rixon F . Measuring the dielectric properties of herpes simplex virus type 1 virions with dielectrophoresis. Biochim Biophys Acta. 2002; 1571(1):1-8. DOI: 10.1016/s0304-4165(02)00161-7. View

Zhang W, Mukhopadhyay S, Pletnev S, Baker T, Kuhn R, Rossmann M . Placement of the structural proteins in Sindbis virus. J Virol. 2002; 76(22):11645-58. PMC: 136788. DOI: 10.1128/jvi.76.22.11645-11658.2002. View

Ermolina I, Morgan H . The electrokinetic properties of latex particles: comparison of electrophoresis and dielectrophoresis. J Colloid Interface Sci. 2005; 285(1):419-28. DOI: 10.1016/j.jcis.2004.11.003. View

Lapizco-Encinas B, Davalos R, Simmons B, Cummings E, Fintschenko Y . An insulator-based (electrodeless) dielectrophoretic concentrator for microbes in water. J Microbiol Methods. 2005; 62(3):317-26. DOI: 10.1016/j.mimet.2005.04.027. View

Grom F, Kentsch J, Muller T, Schnelle T, Stelzle M . Accumulation and trapping of hepatitis A virus particles by electrohydrodynamic flow and dielectrophoresis. Electrophoresis. 2006; 27(7):1386-93. DOI: 10.1002/elps.200500416. View

Charcosset C . Membrane processes in biotechnology: an overview. Biotechnol Adv. 2006; 24(5):482-92. DOI: 10.1016/j.biotechadv.2006.03.002. View

10.

Kalbfuss B, Genzel Y, Wolff M, Zimmermann A, Morenweiser R, Reichl U . Harvesting and concentration of human influenza A virus produced in serum-free mammalian cell culture for the production of vaccines. Biotechnol Bioeng. 2006; 97(1):73-85. DOI: 10.1002/bit.21139. View

11.

Ermolina I, Milner J, Morgan H . Dielectrophoretic investigation of plant virus particles: Cow Pea Mosaic Virus and Tobacco Mosaic Virus. Electrophoresis. 2006; 27(20):3939-48. DOI: 10.1002/elps.200500928. View

12.

Pysher M, Hayes M . Electrophoretic and dielectrophoretic field gradient technique for separating bioparticles. Anal Chem. 2007; 79(12):4552-7. DOI: 10.1021/ac070534j. View

13.

Lapizco-Encinas B, Rito-Palomares M . Dielectrophoresis for the manipulation of nanobioparticles. Electrophoresis. 2007; 28(24):4521-38. DOI: 10.1002/elps.200700303. View

14.

MacCuspie R, Nuraje N, Lee S, Runge A, Matsui H . Comparison of electrical properties of viruses studied by AC capacitance scanning probe microscopy. J Am Chem Soc. 2007; 130(3):887-91. PMC: 3474603. DOI: 10.1021/ja075244z. View

15.

Kalbfuss B, Flockerzi D, Seidel-Morgenstern A, Reichl U . Size-exclusion chromatography as a linear transfer system: purification of human influenza virus as an example. J Chromatogr B Analyt Technol Biomed Life Sci. 2008; 873(1):102-12. DOI: 10.1016/j.jchromb.2008.08.002. View

16.

Hernandez R, Sinodis C, Brown D . Sindbis virus: propagation, quantification, and storage. Curr Protoc Microbiol. 2008; Chapter 15:Unit 15B.1. DOI: 10.1002/9780471729259.mc15b01s00. View

17.

Lien-Anh Nguyen T, Fonseca Tumilasci V, Singhroy D, Arguello M, Hiscott J . The emergence of combinatorial strategies in the development of RNA oncolytic virus therapies. Cell Microbiol. 2009; 11(6):889-97. DOI: 10.1111/j.1462-5822.2009.01317.x. View

18.

Erickson H . Size and shape of protein molecules at the nanometer level determined by sedimentation, gel filtration, and electron microscopy. Biol Proced Online. 2009; 11:32-51. PMC: 3055910. DOI: 10.1007/s12575-009-9008-x. View

19.

Zhang C, Khoshmanesh K, Mitchell A, Kalantar-Zadeh K . Dielectrophoresis for manipulation of micro/nano particles in microfluidic systems. Anal Bioanal Chem. 2009; 396(1):401-20. DOI: 10.1007/s00216-009-2922-6. View

20.

Goldsmith C, Miller S . Modern uses of electron microscopy for detection of viruses. Clin Microbiol Rev. 2009; 22(4):552-63. PMC: 2772359. DOI: 10.1128/CMR.00027-09. View