Planar Photonic Crystal Biosensor for Quantitative Label-Free Cell Attachment Microscopy

Overview

Journal Adv Opt Mater

Date 2016 Feb 16

PMID 26877910

Citations 5

Authors

Weili Chen

Kenneth D Long

Jonas Kurniawan

Margaret Hung

Hojeong Yu

Brendan A Harley

Brian T Cunningham

Affiliations

Soon will be listed here.

Abstract

In this study, a planar-surface photonic crystal (PC) biosensor for quantitative, kinetic, label-free imaging of cell-surface interactions is demonstrated. The planar biosensor surface eliminates external stimuli to the cells caused by substrate topography to more accurately reflect smooth surface environment encountered by many cell types in vitro. Here, a fabrication approach that combines nanoreplica molding and a horizontal dipping process is used to planarize the surface of the PC biosensor. The planar PC biosensor maintains a high detection sensitivity that enables the monitoring of live cell-substrate interactions with spatial resolution sufficient for observing intracellular attachment strength gradients and the extensions of filopodia from the cell body. The evolution of cell morphology during the attachment and spreading process of 3T3 fibroblast cells is compared between planar and grating-structured PC biosensors. The planar surface effectively eliminates the directionally biased cellular attachment behaviors that are observed on the grating-structured surface. This work represents an important step forward in the development of label-free techniques for observing cellular processes without unintended external environmental modulation.

Citing Articles

Detection and Digital Resolution Counting of Nanoparticles with Optical Resonators and Applications in Biosensing.

Aguirre M, Long K, Li N, Manoto S, Cunningham B Chemosensors (Basel). 2024; 6(2).

PMID: 39559408 PMC: 11573240. DOI: 10.3390/chemosensors6020013.

Microscopies Enabled by Photonic Metamaterials.

Xiong Y, Li N, Che C, Wang W, Barya P, Liu W Sensors (Basel). 2022; 22(3).

PMID: 35161831 PMC: 8840465. DOI: 10.3390/s22031086.

Engineered Tissue Models to Replicate Dynamic Interactions within the Hematopoietic Stem Cell Niche.

Gilchrist A, Harley B Adv Healthc Mater. 2021; 11(7):e2102130.

PMID: 34936239 PMC: 8986554. DOI: 10.1002/adhm.202102130.

Near cut-off wavelength operation of resonant waveguide grating biosensors.

Kovacs B, Kraft F, Szabo Z, Nazirizadeh Y, Gerken M, Horvath R Sci Rep. 2021; 11(1):13091.

PMID: 34158570 PMC: 8219702. DOI: 10.1038/s41598-021-92327-4.

Challenges and Opportunities to Harnessing the (Hematopoietic) Stem Cell Niche.

Choi J, Harley B Curr Stem Cell Rep. 2016; 2(1):85-94.

PMID: 27134819 PMC: 4845958. DOI: 10.1007/s40778-016-0031-y.

References

Chen W, Long K, Lu M, Chaudhery V, Yu H, Choi J . Photonic crystal enhanced microscopy for imaging of live cell adhesion. Analyst. 2013; 138(20):5886-94. DOI: 10.1039/c3an01541f. View

Chalut K, Kulangara K, Giacomelli M, Wax A, Leong K . Deformation of stem cell nuclei by nanotopographical cues. Soft Matter. 2011; 6(8):1675-1681. PMC: 3032404. DOI: 10.1039/B921206J. View

Clark P, Connolly P, Curtis A, Dow J, Wilkinson C . Topographical control of cell behaviour: II. Multiple grooved substrata. Development. 1990; 108(4):635-44. DOI: 10.1242/dev.108.4.635. View

Shafiee H, Lidstone E, Jahangir M, Inci F, Hanhauser E, Henrich T . Nanostructured optical photonic crystal biosensor for HIV viral load measurement. Sci Rep. 2014; 4:4116. PMC: 3937800. DOI: 10.1038/srep04116. View

Unfricht D, Colpitts S, Fernandez S, Lynes M . Grating-coupled surface plasmon resonance: a cell and protein microarray platform. Proteomics. 2005; 5(17):4432-42. DOI: 10.1002/pmic.200401314. View

Barczyk M, Carracedo S, Gullberg D . Integrins. Cell Tissue Res. 2009; 339(1):269-80. PMC: 2784866. DOI: 10.1007/s00441-009-0834-6. View

Biela S, Su Y, Spatz J, Kemkemer R . Different sensitivity of human endothelial cells, smooth muscle cells and fibroblasts to topography in the nano-micro range. Acta Biomater. 2009; 5(7):2460-6. DOI: 10.1016/j.actbio.2009.04.003. View

Rosoff W, Urbach J, Esrick M, McAllister R, Richards L, Goodhill G . A new chemotaxis assay shows the extreme sensitivity of axons to molecular gradients. Nat Neurosci. 2004; 7(6):678-82. DOI: 10.1038/nn1259. View

Nikkhah M, Edalat F, Manoucheri S, Khademhosseini A . Engineering microscale topographies to control the cell-substrate interface. Biomaterials. 2012; 33(21):5230-46. PMC: 3619386. DOI: 10.1016/j.biomaterials.2012.03.079. View

10.

Pelham Jr R, Wang Y . Cell locomotion and focal adhesions are regulated by substrate flexibility. Proc Natl Acad Sci U S A. 1998; 94(25):13661-5. PMC: 28362. DOI: 10.1073/pnas.94.25.13661. View

11.

Friedl P . Dynamic imaging of cellular interactions with extracellular matrix. Histochem Cell Biol. 2004; 122(3):183-90. DOI: 10.1007/s00418-004-0682-0. View

12.

Parker K, Ingber D . Extracellular matrix, mechanotransduction and structural hierarchies in heart tissue engineering. Philos Trans R Soc Lond B Biol Sci. 2007; 362(1484):1267-79. PMC: 2440395. DOI: 10.1098/rstb.2007.2114. View

13.

Flemming R, Murphy C, Abrams G, Goodman S, Nealey P . Effects of synthetic micro- and nano-structured surfaces on cell behavior. Biomaterials. 1999; 20(6):573-88. DOI: 10.1016/s0142-9612(98)00209-9. View

14.

Varshney M, Li Y . Interdigitated array microelectrodes based impedance biosensors for detection of bacterial cells. Biosens Bioelectron. 2008; 24(10):2951-60. DOI: 10.1016/j.bios.2008.10.001. View

15.

Moh K, Yuan X, Bu J, Zhu S, Gao B . Surface plasmon resonance imaging of cell-substrate contacts with radially polarized beams. Opt Express. 2008; 16(25):20734-41. PMC: 3767997. DOI: 10.1364/oe.16.020734. View

16.

Chan L, Gosangari S, Watkin K, Cunningham B . A label-free photonic crystal biosensor imaging method for detection of cancer cell cytotoxicity and proliferation. Apoptosis. 2007; 12(6):1061-8. DOI: 10.1007/s10495-006-0031-y. View

17.

Chen C, Mrksich M, Huang S, Whitesides G, Ingber D . Geometric control of cell life and death. Science. 1997; 276(5317):1425-8. DOI: 10.1126/science.276.5317.1425. View

18.

Cunningham B, Li P, Schulz S, Lin B, Baird C, Gerstenmaier J . Label-free assays on the BIND system. J Biomol Screen. 2004; 9(6):481-90. DOI: 10.1177/1087057104267604. View

19.

Walboomers X, Jansen J . Cell and tissue behavior on micro-grooved surfaces. Odontology. 2003; 89(1):2-11. DOI: 10.1007/s10266-001-8178-z. View

20.

Shamah S, Cunningham B . Label-free cell-based assays using photonic crystal optical biosensors. Analyst. 2011; 136(6):1090-102. DOI: 10.1039/c0an00899k. View