Cloning of 1,2-dichloroethane Degradation Genes of Xanthobacter Autotrophicus GJ10 and Expression and Sequencing of the DhlA Gene

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1989 Dec 1

PMID 2687254

Citations 61

Authors

D B Janssen

F Pries

J van der Ploeg

B Kazemier

P Terpstra

B Witholt

Affiliations

Soon will be listed here.

Abstract

A gene bank from the chlorinated hydrocarbon-degrading bacterium Xanthobacter autotrophicus GJ10 was prepared in the broad-host-range cosmid vector pLAFR1. By using mutants impaired in dichloroethane utilization and strains lacking dehalogenase activities, several genes involved in 1,2-dichloroethane metabolism were isolated. The haloalkane dehalogenase gene dhlA was subcloned, and it was efficiently expressed from its own constitutive promoter in strains of a Pseudomonas sp., Escherichia coli, and a Xanthobacter sp. at levels up to 30% of the total soluble cellular protein. A 3-kilobase-pair BamHI DNA fragment on which the dhlA gene is localized was sequenced. The haloalkane dehalogenase gene was identified by the known N-terminal amino acid sequence of its product and found to encode a 310-amino-acid protein of molecular weight 35,143. Upstream of the dehalogenase gene, a good ribosome-binding site and two consensus E. coli promoter sequences were present.

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References

Boyer H . A complementation analysis of the restriction and modification of DNA in Escherichia coli. J Mol Biol. 1969; 41(3):459-72. DOI: 10.1016/0022-2836(69)90288-5. View

Rozeboom H, Kingma J, Janssen D, Dijkstra B . Crystallization of haloalkane dehalogenase from Xanthobacter autotrophicus GJ10. J Mol Biol. 1988; 200(3):611-2. DOI: 10.1016/0022-2836(88)90548-7. View

SANGER F, Nicklen S, Coulson A . DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977; 74(12):5463-7. PMC: 431765. DOI: 10.1073/pnas.74.12.5463. View

Staden R . A new computer method for the storage and manipulation of DNA gel reading data. Nucleic Acids Res. 1980; 8(16):3673-94. PMC: 324183. DOI: 10.1093/nar/8.16.3673. View

Ditta G, Stanfield S, Corbin D, Helinski D . Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti. Proc Natl Acad Sci U S A. 1980; 77(12):7347-51. PMC: 350500. DOI: 10.1073/pnas.77.12.7347. View

GOAD W, Kanehisa M . Pattern recognition in nucleic acid sequences. I. A general method for finding local homologies and symmetries. Nucleic Acids Res. 1982; 10(1):247-63. PMC: 326131. DOI: 10.1093/nar/10.1.247. View

Friedman A, Long S, Brown S, Buikema W, Ausubel F . Construction of a broad host range cosmid cloning vector and its use in the genetic analysis of Rhizobium mutants. Gene. 1982; 18(3):289-96. DOI: 10.1016/0378-1119(82)90167-6. View

Motosugi K, Soda K . Microbial degradation of synthetic organochlorine compounds. Experientia. 1983; 39(11):1214-20. DOI: 10.1007/BF01990358. View

Heinemann F, Ozols J . The covalent structure of hepatic microsomal epoxide hydrolase. II. The complete amino acid sequence. J Biol Chem. 1984; 259(2):797-804. View

10.

Mulligan M, HAWLEY D, Entriken R, McClure W . Escherichia coli promoter sequences predict in vitro RNA polymerase selectivity. Nucleic Acids Res. 1984; 12(1 Pt 2):789-800. PMC: 321093. DOI: 10.1093/nar/12.1part2.789. View

11.

Davison J, Heusterspreute M, MERCHEZ M, Brunel F . Vectors with restriction-site banks. I. pJRD158, a 3903-bp plasmid containing 28 unique cloning sites. Gene. 1984; 28(3):311-8. DOI: 10.1016/0378-1119(84)90148-3. View

12.

Fulton G, Nunn D, Lidstrom M . Molecular cloning of a malyl coenzyme A lyase gene from Pseudomonas sp. strain AM1, a facultative methylotroph. J Bacteriol. 1984; 160(2):718-23. PMC: 214796. DOI: 10.1128/jb.160.2.718-723.1984. View

13.

Lipman D, Pearson W . Rapid and sensitive protein similarity searches. Science. 1985; 227(4693):1435-41. DOI: 10.1126/science.2983426. View

14.

Vieira J, Messing J . Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 1985; 33(1):103-19. DOI: 10.1016/0378-1119(85)90120-9. View

15.

Janssen D, Scheper A, Dijkhuizen L, Witholt B . Degradation of halogenated aliphatic compounds by Xanthobacter autotrophicus GJ10. Appl Environ Microbiol. 1985; 49(3):673-7. PMC: 373569. DOI: 10.1128/aem.49.3.673-677.1985. View

16.

Keuning S, Janssen D, Witholt B . Purification and characterization of hydrolytic haloalkane dehalogenase from Xanthobacter autotrophicus GJ10. J Bacteriol. 1985; 163(2):635-9. PMC: 219169. DOI: 10.1128/jb.163.2.635-639.1985. View

17.

McClure W . Mechanism and control of transcription initiation in prokaryotes. Annu Rev Biochem. 1985; 54:171-204. DOI: 10.1146/annurev.bi.54.070185.001131. View

18.

Nunn D, Lidstrom M . Phenotypic characterization of 10 methanol oxidation mutant classes in Methylobacterium sp. strain AM1. J Bacteriol. 1986; 166(2):591-7. PMC: 214645. DOI: 10.1128/jb.166.2.591-597.1986. View

19.

Jeenes D, Soldati L, Baur H, Watson J, Mercenier A, Reimmann C . Expression of biosynthetic genes from Pseudomonas aeruginosa and Escherichia coli in the heterologous host. Mol Gen Genet. 1986; 203(3):421-9. DOI: 10.1007/BF00422066. View

20.

Janssen D, Jager D, Witholt B . Degradation of n-haloalkanes and alpha, omega-dihaloalkanes by wild-type and mutants of Acinetobacter sp. strain GJ70. Appl Environ Microbiol. 1987; 53(3):561-6. PMC: 203706. DOI: 10.1128/aem.53.3.561-566.1987. View