Fibroblast Growth Factor-10 (FGF-10) Mobilizes Lung-resident Mesenchymal Stem Cells and Protects Against Acute Lung Injury

Overview

Journal Sci Rep

Specialty Science

Date 2016 Feb 13

PMID 26869337

Citations 37

Authors

Lin Tong

Jian Zhou

Linyi Rong

Eric J Seeley

Jue Pan

Xiaodan Zhu

Jie Liu

Qin Wang

Xinjun Tang

Jieming Qu

Chunxue Bai

Yuanlin Song

Affiliations

Soon will be listed here.

Abstract

FGF-10 can prevent or reduce lung specific inflammation due to traumatic or infectious lung injury. However, the exact mechanisms are poorly characterized. Additionally, the effect of FGF-10 on lung-resident mesenchymal stem cells (LR-MSCs) has not been studied. To better characterize the effect of FGF-10 on LR-MSCs, FGF-10 was intratracheally delivered into the lungs of rats. Three days after instillation, bronchoalveolar lavage was performed and plastic-adherent cells were cultured, characterized and then delivered therapeutically to rats after LPS intratracheal instillation. Immunophenotyping analysis of FGF-10 mobilized and cultured cells revealed expression of the MSC markers CD29, CD73, CD90, and CD105, and the absence of the hematopoietic lineage markers CD34 and CD45. Multipotency of these cells was demonstrated by their capacity to differentiate into osteocytes, adipocytes, and chondrocytes. Delivery of LR-MSCs into the lungs after LPS injury reduced the inflammatory response as evidenced by decreased wet-to-dry ratio, reduced neutrophil and leukocyte recruitment and decreased inflammatory cytokines compared to control rats. Lastly, direct delivery of FGF-10 in the lungs of rats led to an increase of LR-MSCs in the treated lungs, suggesting that the protective effect of FGF-10 might be mediated, in part, by the mobilization of LR-MSCs in lungs.

Citing Articles

Human Umbilical Cord-Mesenchymal Stem Cells Combined With Low Dosage Nintedanib Rather Than Using Alone Mitigates Pulmonary Fibrosis in Mice.

Qiu H, Zhang R, Si D, Shu Y, Liu J, Xia Y Stem Cells Int. 2025; 2025:9445735.

PMID: 39817116 PMC: 11732289. DOI: 10.1155/sci/9445735.

Endothelial cell dynamics in sepsis-induced acute lung injury and acute respiratory distress syndrome: pathogenesis and therapeutic implications.

Qiao X, Yin J, Zheng Z, Li L, Feng X Cell Commun Signal. 2024; 22(1):241.

PMID: 38664775 PMC: 11046830. DOI: 10.1186/s12964-024-01620-y.

Stem/Progenitor Cells and Related Therapy in Bronchopulmonary Dysplasia.

Marega M, El-Merhie N, Gokyildirim M, Orth V, Bellusci S, Chao C Int J Mol Sci. 2023; 24(13).

PMID: 37446407 PMC: 10342250. DOI: 10.3390/ijms241311229.

Pulmonary endogenous progenitor stem cell subpopulation: Physiology, pathogenesis, and progress.

Liu D, Xu C, Jiang L, Zhu X J Intensive Med. 2023; 3(1):38-51.

PMID: 36789358 PMC: 9924023. DOI: 10.1016/j.jointm.2022.08.005.

The Role of Lung Resident Mesenchymal Stromal Cells in the Pathogenesis and Repair of Chronic Lung Disease.

Doherty D, Roets L, Krasnodembskaya A Stem Cells. 2023; 41(5):431-443.

PMID: 36749355 PMC: 10183969. DOI: 10.1093/stmcls/sxad014.

References

Beer H, Vindevoghel L, Gait M, Revest J, Duan D, Mason I . Fibroblast growth factor (FGF) receptor 1-IIIb is a naturally occurring functional receptor for FGFs that is preferentially expressed in the skin and the brain. J Biol Chem. 2000; 275(21):16091-7. DOI: 10.1074/jbc.275.21.16091. View

Tong L, Bi J, Zhu X, Wang G, Liu J, Rong L . Keratinocyte growth factor-2 is protective in lipopolysaccharide-induced acute lung injury in rats. Respir Physiol Neurobiol. 2014; 201:7-14. DOI: 10.1016/j.resp.2014.06.011. View

Ware L, Matthay M . Keratinocyte and hepatocyte growth factors in the lung: roles in lung development, inflammation, and repair. Am J Physiol Lung Cell Mol Physiol. 2002; 282(5):L924-40. DOI: 10.1152/ajplung.00439.2001. View

Cras-Meneur C, Scharfmann R . FGFR1-IIIb is a putative marker of pancreatic progenitor cells. Mech Dev. 2002; 116(1-2):205-8. DOI: 10.1016/s0925-4773(02)00138-7. View

Ortiz L, Gambelli F, McBride C, Gaupp D, Baddoo M, Kaminski N . Mesenchymal stem cell engraftment in lung is enhanced in response to bleomycin exposure and ameliorates its fibrotic effects. Proc Natl Acad Sci U S A. 2003; 100(14):8407-11. PMC: 166242. DOI: 10.1073/pnas.1432929100. View

FRIEDENSTEIN A, Chailakhyan R, Gerasimov U . Bone marrow osteogenic stem cells: in vitro cultivation and transplantation in diffusion chambers. Cell Tissue Kinet. 1987; 20(3):263-72. DOI: 10.1111/j.1365-2184.1987.tb01309.x. View

Yamasaki M, Miyake A, Tagashira S, Itoh N . Structure and expression of the rat mRNA encoding a novel member of the fibroblast growth factor family. J Biol Chem. 1996; 271(27):15918-21. DOI: 10.1074/jbc.271.27.15918. View

EMOTO H, Tagashira S, Mattei M, Yamasaki M, Hashimoto G, Katsumata T . Structure and expression of human fibroblast growth factor-10. J Biol Chem. 1997; 272(37):23191-4. DOI: 10.1074/jbc.272.37.23191. View

Igarashi M, Finch P, Aaronson S . Characterization of recombinant human fibroblast growth factor (FGF)-10 reveals functional similarities with keratinocyte growth factor (FGF-7). J Biol Chem. 1998; 273(21):13230-5. DOI: 10.1074/jbc.273.21.13230. View

10.

Sekine K, Ohuchi H, Fujiwara M, Yamasaki M, Yoshizawa T, Sato T . Fgf10 is essential for limb and lung formation. Nat Genet. 1999; 21(1):138-41. DOI: 10.1038/5096. View

11.

Pittenger M, Mackay A, Beck S, Jaiswal R, Douglas R, Mosca J . Multilineage potential of adult human mesenchymal stem cells. Science. 1999; 284(5411):143-7. DOI: 10.1126/science.284.5411.143. View

12.

Su X, Looney M, Robriquet L, Fang X, Matthay M . Direct visual instillation as a method for efficient delivery of fluid into the distal airspaces of anesthetized mice. Exp Lung Res. 2004; 30(6):479-93. DOI: 10.1080/01902140490476382. View

13.

Hyatt B, Shangguan X, Shannon J . FGF-10 induces SP-C and Bmp4 and regulates proximal-distal patterning in embryonic tracheal epithelium. Am J Physiol Lung Cell Mol Physiol. 2004; 287(6):L1116-26. DOI: 10.1152/ajplung.00033.2004. View

14.

Sabatini F, Petecchia L, Tavian M, Jodon de Villeroche V, Rossi G, Brouty-Boye D . Human bronchial fibroblasts exhibit a mesenchymal stem cell phenotype and multilineage differentiating potentialities. Lab Invest. 2005; 85(8):962-71. DOI: 10.1038/labinvest.3700300. View

15.

Dominici M, Le Blanc K, Mueller I, Slaper-Cortenbach I, Marini F, Krause D . Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy. 2006; 8(4):315-7. DOI: 10.1080/14653240600855905. View

16.

Baber S, Deng W, Master R, Bunnell B, Taylor B, Murthy S . Intratracheal mesenchymal stem cell administration attenuates monocrotaline-induced pulmonary hypertension and endothelial dysfunction. Am J Physiol Heart Circ Physiol. 2006; 292(2):H1120-8. DOI: 10.1152/ajpheart.00173.2006. View

17.

Benjamin J, Smith R, Halloran B, Day T, Kelly D, Prince L . FGF-10 is decreased in bronchopulmonary dysplasia and suppressed by Toll-like receptor activation. Am J Physiol Lung Cell Mol Physiol. 2006; 292(2):L550-8. DOI: 10.1152/ajplung.00329.2006. View

18.

Lama V, Smith L, Badri L, Flint A, Andrei A, Murray S . Evidence for tissue-resident mesenchymal stem cells in human adult lung from studies of transplanted allografts. J Clin Invest. 2007; 117(4):989-96. PMC: 1810571. DOI: 10.1172/JCI29713. View

19.

Gupta N, Su X, Popov B, Lee J, Serikov V, Matthay M . Intrapulmonary delivery of bone marrow-derived mesenchymal stem cells improves survival and attenuates endotoxin-induced acute lung injury in mice. J Immunol. 2007; 179(3):1855-63. DOI: 10.4049/jimmunol.179.3.1855. View

20.

Zhen G, Liu H, Gu N, Zhang H, Xu Y, Zhang Z . Mesenchymal stem cells transplantation protects against rat pulmonary emphysema. Front Biosci. 2008; 13:3415-22. DOI: 10.2741/2936. View