Antimicrobial Susceptibility and Molecular Epidemiology of Extended-spectrum Beta-lactamase-producing Enterobacteriaceae from Intensive Care Units at Hamad Medical Corporation, Qatar

Overview

Journal Antimicrob Resist Infect Control

Publisher Biomed Central

Specialty Infectious Diseases

Date 2016 Feb 12

PMID 26865975

Citations 28

Authors

Mazen A Sid Ahmed

Devendra Bansal

Anushree Acharya

Asha A Elmi

Jemal M Hamid

Abuelhassan M Sid Ahmed

Prem Chandra

Emad Ibrahim

Ali A Sultan

Sanjay Doiphode

Naser Eldin Bilal

Anand Deshmukh

Affiliations

Soon will be listed here.

Abstract

Background: The emergence of extended-spectrum beta-lactamase (ESBL)-producing isolates has important clinical and therapeutic implications. High prevalence of ESBL-producing Enterobacteriaceae has been reported in the literature for clinical samples from a variety of infection sites. The present study was undertaken to evaluate the prevalence of ESBL-producing Enterobacteriaceae, and to perform molecular characterization and antimicrobial susceptibility testing of clinical isolates from patients admitted to the intensive care units at Hamad Medical Corporation, Doha, Qatar, from November 2012 to October 2013.

Methods: A total of 629 Enterobacteriaceae isolates were included in the study. Identification and susceptibility testing was performed using Phoenix (Becton Dickinson) and the ESBL producers were confirmed by double-disk potentiation as recommended by the Clinical and Laboratory Standards Institute. Molecular analysis of the ESBL producers was performed by polymerase chain reaction.

Results: In total, 109 isolates (17.3 %) were confirmed as ESBL producers and all were sensitive to meropenem in routine susceptibility assays. Most of the ESBL producers (99.1 %) were resistant to amoxicillin/clavulanic acid and ceftriaxone and 93.6 % were resistant to cefepime. Among the ESBL-producing genes, bla CTX-M (66.1 %) was the most prevalent, followed by bla SHV (53.2 %) and bla TEM (40.4 %).

Conclusions: These findings show the high prevalence of ESBL-producing Enterobacteriaceae within the intensive care units at Hamad Medical Corporation, Qatar, and emphasize the need for judicious use of antibiotics and the implementation of strict infection control measures.

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References

Talbot G, Bradley J, Edwards Jr J, Gilbert D, Scheld M, Bartlett J . Bad bugs need drugs: an update on the development pipeline from the Antimicrobial Availability Task Force of the Infectious Diseases Society of America. Clin Infect Dis. 2006; 42(5):657-68. DOI: 10.1086/499819. View

Canton R, Coque T . The CTX-M beta-lactamase pandemic. Curr Opin Microbiol. 2006; 9(5):466-75. DOI: 10.1016/j.mib.2006.08.011. View

Garza-Gonzalez E, Mendoza Ibarra S, Llaca-Diaz J, Gonzalez G . Molecular characterization and antimicrobial susceptibility of extended-spectrum {beta}-lactamase-producing Enterobacteriaceae isolates at a tertiary-care centre in Monterrey, Mexico. J Med Microbiol. 2010; 60(Pt 1):84-90. DOI: 10.1099/jmm.0.022970-0. View

Somily A, Habib H, Absar M, Arshad M, Manneh K, Al Subaie S . ESBL-producing Escherichia coli and Klebsiella pneumoniae at a tertiary care hospital in Saudi Arabia. J Infect Dev Ctries. 2014; 8(9):1129-36. DOI: 10.3855/jidc.4292. View

Queipo-Ortuno M, Colmenero J, Macias M, Bravo M, Morata P . Preparation of bacterial DNA template by boiling and effect of immunoglobulin G as an inhibitor in real-time PCR for serum samples from patients with brucellosis. Clin Vaccine Immunol. 2007; 15(2):293-6. PMC: 2238042. DOI: 10.1128/CVI.00270-07. View

Zhao W, Hu Z . Epidemiology and genetics of CTX-M extended-spectrum β-lactamases in Gram-negative bacteria. Crit Rev Microbiol. 2012; 39(1):79-101. PMC: 4086240. DOI: 10.3109/1040841X.2012.691460. View

Giriyapur R, Nandihal N, Krishna B, Patil A, Chandrasekhar M . Comparison of disc diffusion methods for the detection of extended-spectrum Beta lactamase-producing enterobacteriaceae. J Lab Physicians. 2011; 3(1):33-6. PMC: 3118054. DOI: 10.4103/0974-2727.78561. View

DAndrea M, Arena F, Pallecchi L, Rossolini G . CTX-M-type β-lactamases: a successful story of antibiotic resistance. Int J Med Microbiol. 2013; 303(6-7):305-17. DOI: 10.1016/j.ijmm.2013.02.008. View

Zowawi H, Balkhy H, Walsh T, Paterson D . β-Lactamase production in key gram-negative pathogen isolates from the Arabian Peninsula. Clin Microbiol Rev. 2013; 26(3):361-80. PMC: 3719487. DOI: 10.1128/CMR.00096-12. View

10.

Lahlaoui H, Ben Haj Khalifa A, Ben Moussa M . Epidemiology of Enterobacteriaceae producing CTX-M type extended spectrum β-lactamase (ESBL). Med Mal Infect. 2014; 44(9):400-4. DOI: 10.1016/j.medmal.2014.03.010. View

11.

Nakamura T, Komatsu M, Yamasaki K, Fukuda S, Miyamoto Y, Higuchi T . Epidemiology of Escherichia coli, Klebsiella species, and Proteus mirabilis strains producing extended-spectrum β-lactamases from clinical samples in the Kinki Region of Japan. Am J Clin Pathol. 2012; 137(4):620-6. DOI: 10.1309/AJCP48PDVKWQOXEZ. View

12.

Hassan H, Abdalhamid B . Molecular characterization of extended-spectrum beta-lactamase producing Enterobacteriaceae in a Saudi Arabian tertiary hospital. J Infect Dev Ctries. 2014; 8(3):282-8. DOI: 10.3855/jidc.3809. View

13.

Yezli S, Shibl A, Memish Z . The molecular basis of β-lactamase production in Gram-negative bacteria from Saudi Arabia. J Med Microbiol. 2014; 64(Pt 2):127-136. DOI: 10.1099/jmm.0.077834-0. View

14.

Giske C, Monnet D, Cars O, Carmeli Y . Clinical and economic impact of common multidrug-resistant gram-negative bacilli. Antimicrob Agents Chemother. 2007; 52(3):813-21. PMC: 2258516. DOI: 10.1128/AAC.01169-07. View

15.

Bush K, Fisher J . Epidemiological expansion, structural studies, and clinical challenges of new β-lactamases from gram-negative bacteria. Annu Rev Microbiol. 2011; 65:455-78. DOI: 10.1146/annurev-micro-090110-102911. View

16.

Shahid M, Singh A, Sobia F, Rashid M, Malik A, Shukla I . bla(CTX-M), bla(TEM), and bla(SHV) in Enterobacteriaceae from North-Indian tertiary hospital: high occurrence of combination genes. Asian Pac J Trop Med. 2011; 4(2):101-5. DOI: 10.1016/S1995-7645(11)60046-1. View

17.

Feizabadi M, Delfani S, Raji N, Majnooni A, Aligholi M, Shahcheraghi F . Distribution of bla(TEM), bla(SHV), bla(CTX-M) genes among clinical isolates of Klebsiella pneumoniae at Labbafinejad Hospital, Tehran, Iran. Microb Drug Resist. 2009; 16(1):49-53. DOI: 10.1089/mdr.2009.0096. View

18.

Mokaddas E, Abdulla A, Shati S, Rotimi V . The technical aspects and clinical significance of detecting extended-spectrum beta-lactamase-producing Enterobacteriaceae at a tertiary-care hospital in Kuwait. J Chemother. 2008; 20(4):445-51. DOI: 10.1179/joc.2008.20.4.445. View

19.

Lewis 2nd J, Herrera M, Wickes B, Patterson J, Jorgensen J . First report of the emergence of CTX-M-type extended-spectrum beta-lactamases (ESBLs) as the predominant ESBL isolated in a U.S. health care system. Antimicrob Agents Chemother. 2007; 51(11):4015-21. PMC: 2151438. DOI: 10.1128/AAC.00576-07. View

20.

Gur D, Hascelik G, Aydin N, Telli M, Gultekin M, Ogulnc D . Antimicrobial resistance in gram-negative hospital isolates: results of the Turkish HITIT-2 Surveillance Study of 2007. J Chemother. 2009; 21(4):383-9. DOI: 10.1179/joc.2009.21.4.383. View