A Murine, Bispecific Monoclonal Antibody Simultaneously Recognizing β-Glucan and MP65 Determinants in Candida Species

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2016 Feb 10

PMID 26859561

Citations 5

Authors

Andrea Zito

Carla Bromuro

Giorgia Mandili

Paola Chiani

Alberto L Horenstein

Fabio Malavasi

Roberto Cauda

Antonio Cassone

Antonella Torosantucci

Affiliations

Soon will be listed here.

Abstract

There is a real medical need of new diagnostic tools for the early recognition of invasive Candida infections. We exploited a rather simple and rapid redox methodology to construct a bispecific monoclonal antibody (bsmAb) that combines a monoclonal antibody (mAb) directed against 1,3-β-D-glucan, a well-known, pan-fungal diagnostic biomarker, with a mAb recognizing MP65, a major immunogenic mannoprotein secreted by C.albicans and other Candida species. The bsmAb (MP65/bglu mAb) was successfully produced and purified at high yields and proved to bind and reveal simultaneously, with high sensitivity, the β-glucan and MP65 antigens in both purified and native forms. The MP65/bglu mAb is the first bispecific antibody generated against a fungal microorganism and may prove useful for the concurrent detection of different and clinically significant Candida biomarkers in patient sera.

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