Cryopreservation of Adipose-Derived Mesenchymal Stem Cells

Overview

Journal Cell Med

Publisher Sage Publications

Date 2016 Feb 10

PMID 26858903

Citations 26

Authors

Chika Miyagi-Shiohira

Kiyoto Kurima

Naoya Kobayashi

Issei Saitoh

Masami Watanabe

Yasufumi Noguchi

Masayuki Matsushita

Hirofumi Noguchi

Affiliations

Soon will be listed here.

Abstract

Mesenchymal stem cells (MSCs) have the potential to differentiate into cells of mesodermal origin such as osteoblasts, adipocytes, myocytes, and chondrocytes. They possess an immunosuppressive effect, which makes them a viable cell population for the cell-based therapy of treatment-resistant immune diseases. Adipose-derived mesenchymal stem cells (ASCs) have been demonstrated to have the ability to acquire the properties of subcutaneous adipose tissue particularly easily, and cryopreservation is currently performed as a routine method for preserving ASCs to safely acquire large numbers of cells. However, many studies have reported that cellular activity after freezing and thawing may be affected by the solutions used for cryopreservation. Dimethyl sulfoxide (DMSO) is commonly used as a cryopreservation medium as it diffuses into the cell through the plasma membrane and protects the cells from the damage caused by freezing. As substitutes for DMSO or animal-derived serum, cell banker series, polyvinylpyrrolidone (PVP), sericin and maltose, and methyl cellulose (MC) have been investigated for their clinical applications. It is critical to develop a reliable cell cryopreservation protocol for regenerative medicine using MSCs.

Citing Articles

Distinct subpopulations of human subcutaneous adipose tissue precursor cells revealed by single-cell RNA sequencing.

Divoux A, Whytock K, Halasz L, Hopf M, Sparks L, Osborne T Am J Physiol Cell Physiol. 2024; 326(4):C1248-C1261.

PMID: 38581663 PMC: 11193533. DOI: 10.1152/ajpcell.00726.2023.

Transplantation of adipose derived stem cells in diabetes mellitus; limitations and achievements.

Tajali R, Eidi A, Tafti H, Pazouki A, Kamarul T, Sharifi A J Diabetes Metab Disord. 2023; 22(2):1039-1052.

PMID: 37975135 PMC: 10638327. DOI: 10.1007/s40200-023-01280-8.

Factors Defining Human Adipose Stem/Stromal Cell Immunomodulation in Vitro.

Mahmoud M, Abdel-Rasheed M, Galal E, R El-Awady R Stem Cell Rev Rep. 2023; 20(1):175-205.

PMID: 37962697 PMC: 10799834. DOI: 10.1007/s12015-023-10654-7.

SVF-GEL Cryopreserved for Different Times Exhibits Varied Preservation and Regeneration Potential After Transplantation in a Mouse Model.

Tao Y, Zhao Z, Xiang X, Liang Z, Zhao Y Aesthetic Plast Surg. 2022; 47(2):842-851.

PMID: 36074301 PMC: 10070215. DOI: 10.1007/s00266-022-03065-5.

Biomaterials directed activation of a cryostable therapeutic secretome in induced pluripotent stem cell derived mesenchymal stromal cells.

Romanazzo S, Kopecky C, Jiang S, Doshi R, Mukund V, Srivastava P J Tissue Eng Regen Med. 2022; 16(11):1008-1018.

PMID: 36017672 PMC: 9804847. DOI: 10.1002/term.3347.

References

Hass R, Kasper C, Bohm S, Jacobs R . Different populations and sources of human mesenchymal stem cells (MSC): A comparison of adult and neonatal tissue-derived MSC. Cell Commun Signal. 2011; 9:12. PMC: 3117820. DOI: 10.1186/1478-811X-9-12. View

Minonzio G, Corazza M, Mariotta L, Gola M, Zanzi M, Gandolfi E . Frozen adipose-derived mesenchymal stem cells maintain high capability to grow and differentiate. Cryobiology. 2014; 69(2):211-6. DOI: 10.1016/j.cryobiol.2014.07.005. View

Oishi K, Noguchi H, Yukawa H, Miyazaki T, Kato R, Kitagawa Y . Cryopreservation of mouse adipose tissue-derived stem/progenitor cells. Cell Transplant. 2008; 17(1-2):35-41. DOI: 10.3727/000000008783906937. View

Ohgushi H, Caplan A . Stem cell technology and bioceramics: from cell to gene engineering. J Biomed Mater Res. 1999; 48(6):913-27. DOI: 10.1002/(sici)1097-4636(1999)48:6<913::aid-jbm22>3.0.co;2-0. View

Meryman H . Cryopreservation of living cells: principles and practice. Transfusion. 2007; 47(5):935-45. DOI: 10.1111/j.1537-2995.2007.01212.x. View

Noguchi H . Stem cell applications in diabetes. J Stem Cells. 2013; 7(4):229-44. DOI: jsc.2013.7.4.229. View

MATHE G, JAMMET H, PENDIC B, SCHWARZENBERG L, DUPLAN J, MAUPIN B . [Transfusions and grafts of homologous bone marrow in humans after accidental high dosage irradiation]. Rev Fr Etud Clin Biol. 1959; 4(3):226-38. View

Noguchi H . Production of pancreatic beta-cells from stem cells. Curr Diabetes Rev. 2010; 6(3):184-90. DOI: 10.2174/157339910791162934. View

MATHE G, Bernard J, SCHWARZENBERG L, LARRIEU M, LALANNE C, Dutreix A . [Trial treatment of patients afflicted with acute leukemia in remission with total irradiation followed by homologous bone marrow transfusion]. Rev Fr Etud Clin Biol. 1959; 4:675-704. View

10.

Al-Saqi S, Saliem M, Quezada H, Ekblad A, Jonasson A, Hovatta O . Defined serum- and xeno-free cryopreservation of mesenchymal stem cells. Cell Tissue Bank. 2014; 16(2):181-93. DOI: 10.1007/s10561-014-9463-8. View

11.

King S, Hanson S, Hematti P, Thibeault S . Current applications of mesenchymal stem cells for tissue replacement in otolaryngology-head and neck surgery. Am J Stem Cells. 2013; 1(3):225-38. PMC: 3636735. View

12.

Gonda K, Shigeura T, Sato T, Matsumoto D, Suga H, Inoue K . Preserved proliferative capacity and multipotency of human adipose-derived stem cells after long-term cryopreservation. Plast Reconstr Surg. 2008; 121(2):401-410. DOI: 10.1097/01.prs.0000298322.70032.bc. View

13.

Higman M, Port J, Beauchamp Jr N, Chen A . Reversible leukoencephalopathy associated with re-infusion of DMSO preserved stem cells. Bone Marrow Transplant. 2000; 26(7):797-800. DOI: 10.1038/sj.bmt.1702589. View

14.

Windrum P, Morris T, Drake M, Niederwieser D, Ruutu T . Variation in dimethyl sulfoxide use in stem cell transplantation: a survey of EBMT centres. Bone Marrow Transplant. 2005; 36(7):601-3. DOI: 10.1038/sj.bmt.1705100. View

15.

Liu G, Zhou H, Li Y, Li G, Cui L, Liu W . Evaluation of the viability and osteogenic differentiation of cryopreserved human adipose-derived stem cells. Cryobiology. 2008; 57(1):18-24. DOI: 10.1016/j.cryobiol.2008.04.002. View

16.

Ohno T, Kurita K, Abe S, Eimori N, Ikawa Y . A simple freezing medium for serum-free cultured cells. Cytotechnology. 2012; 1(3):257-60. DOI: 10.1007/BF00145029. View

17.

Gimble J, Katz A, Bunnell B . Adipose-derived stem cells for regenerative medicine. Circ Res. 2007; 100(9):1249-60. PMC: 5679280. DOI: 10.1161/01.RES.0000265074.83288.09. View

18.

Dominici M, Le Blanc K, Mueller I, Slaper-Cortenbach I, Marini F, Krause D . Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy. 2006; 8(4):315-7. DOI: 10.1080/14653240600855905. View

19.

Thirumala S, Wu X, Gimble J, Devireddy R . Evaluation of polyvinylpyrrolidone as a cryoprotectant for adipose tissue-derived adult stem cells. Tissue Eng Part C Methods. 2009; 16(4):783-92. DOI: 10.1089/ten.TEC.2009.0552. View

20.

Noguchi H . Stem cells for the treatment of diabetes. Endocr J. 2006; 54(1):7-16. DOI: 10.1507/endocrj.kr-86. View