Annexin A6 Regulates Interleukin-2-mediated T-cell Proliferation

Overview

Journal Immunol Cell Biol

Publisher Wiley

Specialties Allergy & Immunology
Cell Biology

Date 2016 Feb 9

PMID 26853809

Citations 16

Authors

Rhea Cornely

Abigail H Pollock

Carles Rentero

Sarah E Norris

Anna Alvarez-Guaita

Thomas Grewal

Todd Mitchell

Carlos Enrich

Stephen E Moss

Robert G Parton

Jeremie Rossy

Katharina Gaus

Affiliations

Soon will be listed here.

Abstract

Annexin A6 (AnxA6) has been implicated in cell signalling by contributing to the organisation of the plasma membrane. Here we examined whether AnxA6 regulates signalling and proliferation in T cells. We used a contact hypersensitivity model to immune challenge wild-type (WT) and AnxA6(-/-) mice and found that the in vivo proliferation of CD4(+) T cells, but not CD8(+) T cells, was impaired in AnxA6(-/-) relative to WT mice. However, T-cell migration and signalling through the T-cell receptor ex vivo was similar between T cells isolated from AnxA6(-/-) and WT mice. In contrast, interleukin-2 (IL-2) signalling was reduced in AnxA6(-/-) compared with WT T cells. Further, AnxA6-deficient T cells had reduced membrane order and cholesterol levels. Taken together, our data suggest that AnxA6 regulates IL-2 homeostasis and sensitivity in T cells by sustaining a lipid raft-like membrane environment.

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