Expression of Autophagy and Reactive Oxygen Species-Related Proteins in Lacrimal Gland Adenoid Cystic Carcinoma

Overview

Journal Yonsei Med J

Specialty General Medicine

Date 2016 Feb 6

PMID 26847304

Citations 3

Authors

Ja Seung Koo

Ji Won Kim

Jin Sook Yoon

Affiliations

Soon will be listed here.

Abstract

Purpose: To investigate the difference of expression of autophagy and reactive oxygen species (ROS) related proteins in adenoid cystic carcinoma (ACC) of lacrimal gland in comparison with ACC of salivary gland.

Materials And Methods: Formalin-fixed, paraffin-embedded tissue samples from patients pathologically diagnosed as lacrimal gland ACC (n=11) and salivary gland ACC (n=64) were used. Immunochemistry was used to measure expression of autophagy related proteins [beclin-1, light chain (LC) 3A, LC3B, p62, and BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 (BNIP3)] and ROS related proteins [catalase, thioredoxinreductase, glutathione S-transferasepi (GSTpi), thioredoxin interacting protein, and manganese superoxide dismutase (MnSOD)]. The prognostic factors related to disease-free and overall survival (OS) in lacrimal gland ACC by log-rank tests, were determined.

Results: GSTpi in stromal cells was more highly expressed in lacrimal gland ACC (p=0.006), however, MnSOD in epithelial cells was expressed more in salivary gland ACC (p=0.046). LC3B positivity and BNIP3 positivity in epithelial component were associated with shorter disease-free survival (both p=0.002), and LC3A positivity in stromal component was the factor related to shorter OS (p=0.005).

Conclusion: This is the first study to demonstrate the expression of autophagy and ROS related proteins in lacrimal gland ACC in comparison with the salivary gland ACC, which would provide a basis for further study of autophagy and ROS mechanism as novel therapeutic targets in lacrimal gland ACC.

Citing Articles

Mitophagy in Cancer: A Tale of Adaptation.

Vara-Perez M, Felipe-Abrio B, Agostinis P Cells. 2019; 8(5).

PMID: 31121959 PMC: 6562743. DOI: 10.3390/cells8050493.

Cytoplasmic SQSTM1/ P62 Accumulation Predicates a Poor Prognosis in Patients with Malignant Tumor.

Zhu L, Wang Y, He J, Tang J, Lv W, Hu J J Cancer. 2018; 9(21):4072-4086.

PMID: 30410612 PMC: 6218778. DOI: 10.7150/jca.26399.

Rasfonin promotes autophagy and apoptosis via upregulation of reactive oxygen species (ROS)/JNK pathway.

Wang W, Sun H, Che Y, Jiang X Mycology. 2018; 7(2):64-73.

PMID: 30123617 PMC: 6059153. DOI: 10.1080/21501203.2016.1170073.

References

Karpathiou G, Sivridis E, Koukourakis M, Mikroulis D, Bouros D, Froudarakis M . Autophagy and Bcl-2/BNIP3 death regulatory pathway in non-small cell lung carcinomas. APMIS. 2012; 121(7):592-604. DOI: 10.1111/apm.12026. View

Oberley L, Buettner G . Role of superoxide dismutase in cancer: a review. Cancer Res. 1979; 39(4):1141-9. View

Zhao H, Yang M, Zhao J, Wang J, Zhang Y, Zhang Q . High expression of LC3B is associated with progression and poor outcome in triple-negative breast cancer. Med Oncol. 2013; 30(1):475. DOI: 10.1007/s12032-013-0475-1. View

Matsuba H, Simpson J, Mauney M, Thawley S . Adenoid cystic salivary gland carcinoma: a clinicopathologic correlation. Head Neck Surg. 1986; 8(3):200-4. DOI: 10.1002/hed.2890080312. View

Wang Y, Zhang W, He K, Liu B, Zhang L, Zhang W . Induction of autophagy-dependent cell death by the survivin suppressant YM155 in salivary adenoid cystic carcinoma. Apoptosis. 2013; 19(4):748-58. PMC: 4211076. DOI: 10.1007/s10495-013-0960-1. View

Polito E, Leccisotti A . Epithelial malignancies of the lacrimal gland: survival rates after extensive and conservative therapy. Ann Ophthalmol. 1993; 25(11):422-6. View

Spowart J, Townsend K, Huwait H, Eshragh S, West N, Ries J . The autophagy protein LC3A correlates with hypoxia and is a prognostic marker of patient survival in clear cell ovarian cancer. J Pathol. 2012; 228(4):437-47. DOI: 10.1002/path.4090. View

Van Emburgh B, Hu J, Levine E, Mosley L, Case L, Lin H . Polymorphisms in drug metabolism genes, smoking, and p53 mutations in breast cancer. Mol Carcinog. 2007; 47(2):88-99. PMC: 3722359. DOI: 10.1002/mc.20365. View

Jiang L, Huang S, Zhang D, Zhang B, Li K, Li W . Inhibition of autophagy augments chemotherapy in human salivary adenoid cystic carcinoma. J Oral Pathol Med. 2013; 43(4):265-72. DOI: 10.1111/jop.12124. View

10.

Huang J, Tan P, Thiyagarajan J, Bay B . Prognostic significance of glutathione S-transferase-pi in invasive breast cancer. Mod Pathol. 2003; 16(6):558-65. DOI: 10.1097/01.MP.0000071842.83169.5A. View

11.

Abd El-Rehim D, Ball G, Pinder S, Rakha E, Paish C, Robertson J . High-throughput protein expression analysis using tissue microarray technology of a large well-characterised series identifies biologically distinct classes of breast cancer confirming recent cDNA expression analyses. Int J Cancer. 2005; 116(3):340-50. DOI: 10.1002/ijc.21004. View

12.

Jiang L, Huang S, Zhang D, Zhang S, Li W, Zheng P . Expression of beclin 1 in primary salivary adenoid cystic carcinoma and its relation to Bcl-2 and p53 and prognosis. Braz J Med Biol Res. 2014; 47(3):252-8. PMC: 3982947. DOI: 10.1590/1414-431X20133231. View

13.

Mulcahy Levy J, Thorburn A . Targeting autophagy during cancer therapy to improve clinical outcomes. Pharmacol Ther. 2011; 131(1):130-41. PMC: 3539744. DOI: 10.1016/j.pharmthera.2011.03.009. View

14.

Prokopakis E, Snyderman C, Hanna E, Carrau R, Johnson J, DAmico F . Risk factors for local recurrence of adenoid cystic carcinoma: the role of postoperative radiation therapy. Am J Otolaryngol. 1999; 20(5):281-6. DOI: 10.1016/s0196-0709(99)90028-5. View

15.

Gibson S . A matter of balance between life and death: targeting reactive oxygen species (ROS)-induced autophagy for cancer therapy. Autophagy. 2010; 6(7):835-7. DOI: 10.4161/auto.6.7.13335. View

16.

Azad M, Chen Y, Gibson S . Regulation of autophagy by reactive oxygen species (ROS): implications for cancer progression and treatment. Antioxid Redox Signal. 2008; 11(4):777-90. DOI: 10.1089/ars.2008.2270. View

17.

Brockstedt U, Krajinovic M, Richer C, Mathonnet G, Sinnett D, Pfau W . Analyses of bulky DNA adduct levels in human breast tissue and genetic polymorphisms of cytochromes P450 (CYPs), myeloperoxidase (MPO), quinone oxidoreductase (NQO1), and glutathione S-transferases (GSTs). Mutat Res. 2002; 516(1-2):41-7. DOI: 10.1016/s1383-5718(02)00019-0. View

18.

Dewaele M, Maes H, Agostinis P . ROS-mediated mechanisms of autophagy stimulation and their relevance in cancer therapy. Autophagy. 2010; 6(7):838-54. DOI: 10.4161/auto.6.7.12113. View

19.

Khan A, DiGiovanna M, Ross D, Sasaki C, Carter D, Son Y . Adenoid cystic carcinoma: a retrospective clinical review. Int J Cancer. 2001; 96(3):149-58. DOI: 10.1002/ijc.1013. View

20.

Van Driel B, Lyon H, Hoogenraad D, Anten S, Hansen U, Van Noorden C . Expression of CuZn- and Mn-superoxide dismutase in human colorectal neoplasms. Free Radic Biol Med. 1997; 23(3):435-44. DOI: 10.1016/s0891-5849(97)00102-0. View