Comparative Fiber Property and Transcriptome Analyses Reveal Key Genes Potentially Related to High Fiber Strength in Cotton (Gossypium Hirsutum L.) Line MD52ne

Overview

Journal BMC Plant Biol

Publisher Biomed Central

Specialty Biology

Date 2016 Feb 3

PMID 26833213

Citations 26

Authors

Md S Islam

David D Fang

Gregory N Thyssen

Chris D Delhom

Yongliang Liu

Hee Jin Kim

Affiliations

Soon will be listed here.

Abstract

Background: Individual fiber strength is an important quality attribute that greatly influences the strength of the yarn spun from cotton fibers. Fiber strength is usually measured from bundles of fibers due to the difficulty of reliably measuring strength from individual cotton fibers. However, bundle fiber strength (BFS) is not always correlated with yarn strength since it is affected by multiple fiber properties involved in fiber-to-fiber interactions within a bundle in addition to the individual fiber strength. Molecular mechanisms responsible for regulating individual fiber strength remain unknown. Gossypium hirsutum near isogenic lines (NILs), MD52ne and MD90ne showing variations in BFS provide an opportunity for dissecting the regulatory mechanisms involved in individual fiber strength.

Results: Comprehensive fiber property analyses of the NILs revealed that the superior bundle strength of MD52ne fibers resulted from high individual fiber strength with minor contributions from greater fiber length. Comparative transcriptome analyses of the NILs showed that the superior bundle strength of MD52ne fibers was potentially related to two signaling pathways: one is ethylene and the interconnected phytohormonal pathways that are involved in cotton fiber elongation, and the other is receptor-like kinases (RLKs) signaling pathways that are involved in maintaining cell wall integrity. Multiple RLKs were differentially expressed in MD52ne fibers and localized in genomic regions encompassing the strength quantitative trait loci (QTLs). Several candidate genes involved in crystalline cellulose assembly were also up-regulated in MD52ne fibers while the secondary cell wall was produced.

Conclusion: Comparative phenotypic and transcriptomic analyses revealed differential expressions of the genes involved in crystalline cellulose assembly, ethylene and RLK signaling pathways between the MD52ne and MD90ne developing fibers. Ethylene and its phytohormonal network might promote the elongation of MD52ne fibers and indirectly contribute to the bundle strength by potentially improving fiber-to-fiber interactions. RLKs that were suggested to mediate a coordination of cell elongation and SCW biosynthesis in other plants might be candidate genes for regulating cotton fiber cell wall assembly and strength.

Citing Articles

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References

Liu Y, Thibodeaux D, Gamble G, Bauer P, VanDerveer D . Comparative investigation of Fourier transform infrared (FT-IR) spectroscopy and X-ray diffraction (XRD) in the determination of cotton fiber crystallinity. Appl Spectrosc. 2012; 66(8):983-6. DOI: 10.1366/12-06611. View

Ching A, Dhugga K, Appenzeller L, Meeley R, Bourett T, Howard R . Brittle stalk 2 encodes a putative glycosylphosphatidylinositol-anchored protein that affects mechanical strength of maize tissues by altering the composition and structure of secondary cell walls. Planta. 2006; 224(5):1174-84. DOI: 10.1007/s00425-006-0299-8. View

Hulsen T, de Vlieg J, Alkema W . BioVenn - a web application for the comparison and visualization of biological lists using area-proportional Venn diagrams. BMC Genomics. 2008; 9:488. PMC: 2584113. DOI: 10.1186/1471-2164-9-488. View

Hinchliffe D, Meredith W, Yeater K, Kim H, Woodward A, Chen Z . Near-isogenic cotton germplasm lines that differ in fiber-bundle strength have temporal differences in fiber gene expression patterns as revealed by comparative high-throughput profiling. Theor Appl Genet. 2010; 120(7):1347-66. DOI: 10.1007/s00122-010-1260-6. View

Singh B, Avci U, Eichler Inwood S, Grimson M, Landgraf J, Mohnen D . A specialized outer layer of the primary cell wall joins elongating cotton fibers into tissue-like bundles. Plant Physiol. 2009; 150(2):684-99. PMC: 2689960. DOI: 10.1104/pp.109.135459. View

Liu L, Shang-Guan K, Zhang B, Liu X, Yan M, Zhang L . Brittle Culm1, a COBRA-like protein, functions in cellulose assembly through binding cellulose microfibrils. PLoS Genet. 2013; 9(8):e1003704. PMC: 3749933. DOI: 10.1371/journal.pgen.1003704. View

Du Z, Zhou X, Ling Y, Zhang Z, Su Z . agriGO: a GO analysis toolkit for the agricultural community. Nucleic Acids Res. 2010; 38(Web Server issue):W64-70. PMC: 2896167. DOI: 10.1093/nar/gkq310. View

Thimm O, Blasing O, Gibon Y, Nagel A, Meyer S, Kruger P . MAPMAN: a user-driven tool to display genomics data sets onto diagrams of metabolic pathways and other biological processes. Plant J. 2004; 37(6):914-39. DOI: 10.1111/j.1365-313x.2004.02016.x. View

Shi Y, Zhu S, Mao X, Feng J, Qin Y, Zhang L . Transcriptome profiling, molecular biological, and physiological studies reveal a major role for ethylene in cotton fiber cell elongation. Plant Cell. 2006; 18(3):651-64. PMC: 1383640. DOI: 10.1105/tpc.105.040303. View

10.

Robinson M, McCarthy D, Smyth G . edgeR: a Bioconductor package for differential expression analysis of digital gene expression data. Bioinformatics. 2009; 26(1):139-40. PMC: 2796818. DOI: 10.1093/bioinformatics/btp616. View

11.

Lee J, Burns T, Light G, Sun Y, Fokar M, Kasukabe Y . Xyloglucan endotransglycosylase/hydrolase genes in cotton and their role in fiber elongation. Planta. 2010; 232(5):1191-205. DOI: 10.1007/s00425-010-1246-2. View

12.

Li F, Fan G, Lu C, Xiao G, Zou C, Kohel R . Genome sequence of cultivated Upland cotton (Gossypium hirsutum TM-1) provides insights into genome evolution. Nat Biotechnol. 2015; 33(5):524-30. DOI: 10.1038/nbt.3208. View

13.

Liu Q, Talbot M, Llewellyn D . Pectin methylesterase and pectin remodelling differ in the fibre walls of two gossypium species with very different fibre properties. PLoS One. 2013; 8(6):e65131. PMC: 3673955. DOI: 10.1371/journal.pone.0065131. View

14.

De Smet I, Voss U, Jurgens G, Beeckman T . Receptor-like kinases shape the plant. Nat Cell Biol. 2009; 11(10):1166-73. DOI: 10.1038/ncb1009-1166. View

15.

Ben-Tov D, Abraham Y, Stav S, Thompson K, Loraine A, Elbaum R . COBRA-LIKE2, a member of the glycosylphosphatidylinositol-anchored COBRA-LIKE family, plays a role in cellulose deposition in arabidopsis seed coat mucilage secretory cells. Plant Physiol. 2015; 167(3):711-24. PMC: 4347734. DOI: 10.1104/pp.114.240671. View

16.

Kim H, Hinchliffe D, Triplett B, Chen Z, Stelly D, Yeater K . Phytohormonal networks promote differentiation of fiber initials on pre-anthesis cotton ovules grown in vitro and in planta. PLoS One. 2015; 10(4):e0125046. PMC: 4415818. DOI: 10.1371/journal.pone.0125046. View

17.

Binder B, Walker J, Gagne J, Emborg T, Hemmann G, Bleecker A . The Arabidopsis EIN3 binding F-Box proteins EBF1 and EBF2 have distinct but overlapping roles in ethylene signaling. Plant Cell. 2007; 19(2):509-23. PMC: 1867343. DOI: 10.1105/tpc.106.048140. View

18.

Mutwil M, Klie S, Tohge T, Giorgi F, Wilkins O, Campbell M . PlaNet: combined sequence and expression comparisons across plant networks derived from seven species. Plant Cell. 2011; 23(3):895-910. PMC: 3082271. DOI: 10.1105/tpc.111.083667. View

19.

Bravo J, Campo S, Murillo I, Coca M, San Segundo B . Fungus- and wound-induced accumulation of mRNA containing a class II chitinase of the pathogenesis-related protein 4 (PR-4) family of maize. Plant Mol Biol. 2003; 52(4):745-59. DOI: 10.1023/a:1025016416951. View

20.

Li G, Meng X, Wang R, Mao G, Han L, Liu Y . Dual-level regulation of ACC synthase activity by MPK3/MPK6 cascade and its downstream WRKY transcription factor during ethylene induction in Arabidopsis. PLoS Genet. 2012; 8(6):e1002767. PMC: 3386168. DOI: 10.1371/journal.pgen.1002767. View