Arabidopsis WRKY6 Transcription Factor Acts As a Positive Regulator of Abscisic Acid Signaling During Seed Germination and Early Seedling Development

Overview

Journal PLoS Genet

Specialty Genetics

Date 2016 Feb 2

PMID 26829043

Citations 63

Authors

Yun Huang

Cui-Zhu Feng

Qing Ye

Wei-Hua Wu

Yi-Fang Chen

Affiliations

Soon will be listed here.

Abstract

The phytohormone abscisic acid (ABA) plays important roles during seed germination and early seedling development. Here, we characterized the function of the Arabidopsis WRKY6 transcription factor in ABA signaling. The transcript of WRKY6 was repressed during seed germination and early seedling development, and induced by exogenous ABA. The wrky6-1 and wrky6-2 mutants were ABA insensitive, whereas WRKY6-overexpressing lines showed ABA-hypersensitive phenotypes during seed germination and early seedling development. The expression of RAV1 was suppressed in the WRKY6-overexpressing lines and elevated in the wrky6 mutants, and the expression of ABI3, ABI4, and ABI5, which was directly down-regulated by RAV1, was enhanced in the WRKY6-overexpressing lines and repressed in the wrky6 mutants. Electrophoretic mobility shift and chromatin immunoprecipitation assays showed that WRKY6 could bind to the RAV1 promoter in vitro and in vivo. Overexpression of RAV1 in WRKY6-overexpressing lines abolished their ABA-hypersensitive phenotypes, and the rav1 wrky6-2 double mutant showed an ABA-hypersensitive phenotype, similar to rav1 mutant. Together, the results demonstrated that the Arabidopsis WRKY6 transcription factor played important roles in ABA signaling by directly down-regulating RAV1 expression.

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References

Finkelstein R, Lynch T . Abscisic acid inhibition of radicle emergence but not seedling growth is suppressed by sugars. Plant Physiol. 2000; 122(4):1179-86. PMC: 58952. DOI: 10.1104/pp.122.4.1179. View

Kasajima I, Ide Y, Hirai M, Fujiwara T . WRKY6 is involved in the response to boron deficiency in Arabidopsis thaliana. Physiol Plant. 2010; 139(1):80-92. DOI: 10.1111/j.1399-3054.2010.01349.x. View

Eulgem T, Rushton P, Robatzek S, Somssich I . The WRKY superfamily of plant transcription factors. Trends Plant Sci. 2000; 5(5):199-206. DOI: 10.1016/s1360-1385(00)01600-9. View

Lopez-Molina L, Chua N . A null mutation in a bZIP factor confers ABA-insensitivity in Arabidopsis thaliana. Plant Cell Physiol. 2000; 41(5):541-7. DOI: 10.1093/pcp/41.5.541. View

Uno Y, Furihata T, Abe H, Yoshida R, Shinozaki K, Yamaguchi-Shinozaki K . Arabidopsis basic leucine zipper transcription factors involved in an abscisic acid-dependent signal transduction pathway under drought and high-salinity conditions. Proc Natl Acad Sci U S A. 2000; 97(21):11632-7. PMC: 17252. DOI: 10.1073/pnas.190309197. View

Robatzek S, Somssich I . A new member of the Arabidopsis WRKY transcription factor family, AtWRKY6, is associated with both senescence- and defence-related processes. Plant J. 2001; 28(2):123-33. DOI: 10.1046/j.1365-313x.2001.01131.x. View

Livak K, Schmittgen T . Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 2002; 25(4):402-8. DOI: 10.1006/meth.2001.1262. View

Choi H, Im M, Kim S . Arabidopsis basic leucine zipper proteins that mediate stress-responsive abscisic acid signaling. Plant Cell. 2002; 14(2):343-57. PMC: 152917. DOI: 10.1105/tpc.010362. View

Robatzek S, Somssich I . Targets of AtWRKY6 regulation during plant senescence and pathogen defense. Genes Dev. 2002; 16(9):1139-49. PMC: 186251. DOI: 10.1101/gad.222702. View

10.

Finkelstein R, Gampala S, Rock C . Abscisic acid signaling in seeds and seedlings. Plant Cell. 2002; 14 Suppl:S15-45. PMC: 151246. DOI: 10.1105/tpc.010441. View

11.

Hu Y, Wang Y, Liu X, Li J . Arabidopsis RAV1 is down-regulated by brassinosteroid and may act as a negative regulator during plant development. Cell Res. 2004; 14(1):8-15. DOI: 10.1038/sj.cr.7290197. View

12.

Ali-Rachedi S, Bouinot D, Wagner M, Bonnet M, Sotta B, Grappin P . Changes in endogenous abscisic acid levels during dormancy release and maintenance of mature seeds: studies with the Cape Verde Islands ecotype, the dormant model of Arabidopsis thaliana. Planta. 2004; 219(3):479-88. DOI: 10.1007/s00425-004-1251-4. View

13.

Ulker B, Somssich I . WRKY transcription factors: from DNA binding towards biological function. Curr Opin Plant Biol. 2004; 7(5):491-8. DOI: 10.1016/j.pbi.2004.07.012. View

14.

Kim S, Kang J, Cho D, Park J, Kim S . ABF2, an ABRE-binding bZIP factor, is an essential component of glucose signaling and its overexpression affects multiple stress tolerance. Plant J. 2004; 40(1):75-87. DOI: 10.1111/j.1365-313X.2004.02192.x. View

15.

Giraudat J, Hauge B, Valon C, Smalle J, Parcy F, Goodman H . Isolation of the Arabidopsis ABI3 gene by positional cloning. Plant Cell. 1992; 4(10):1251-61. PMC: 160212. DOI: 10.1105/tpc.4.10.1251. View

16.

Gaubier P, Raynal M, Hull G, Huestis G, Grellet F, Arenas C . Two different Em-like genes are expressed in Arabidopsis thaliana seeds during maturation. Mol Gen Genet. 1993; 238(3):409-18. DOI: 10.1007/BF00292000. View

17.

Finkelstein R, Wang M, Lynch T, Rao S, Goodman H . The Arabidopsis abscisic acid response locus ABI4 encodes an APETALA 2 domain protein. Plant Cell. 1998; 10(6):1043-54. PMC: 144030. DOI: 10.1105/tpc.10.6.1043. View

18.

Gubler F, Millar A, Jacobsen J . Dormancy release, ABA and pre-harvest sprouting. Curr Opin Plant Biol. 2005; 8(2):183-7. DOI: 10.1016/j.pbi.2005.01.011. View

19.

Fujita Y, Fujita M, Satoh R, Maruyama K, Parvez M, Seki M . AREB1 is a transcription activator of novel ABRE-dependent ABA signaling that enhances drought stress tolerance in Arabidopsis. Plant Cell. 2005; 17(12):3470-88. PMC: 1315382. DOI: 10.1105/tpc.105.035659. View

20.

Fujii H, Verslues P, Zhu J . Identification of two protein kinases required for abscisic acid regulation of seed germination, root growth, and gene expression in Arabidopsis. Plant Cell. 2007; 19(2):485-94. PMC: 1867333. DOI: 10.1105/tpc.106.048538. View