A SNP in Pri-miR-10a is Associated with Recurrent Spontaneous Abortion in a Han-Chinese Population

Overview

Journal Oncotarget

Specialty Oncology

Date 2016 Jan 30

PMID 26824181

Citations 8

Authors

Ying Li

Xue-Qin Wang

Lu Zhang

Xiao-Dan Lv

Xing Su

Shi Tian

Chun-Mei Liu

Xu Ma

Hong-Fei Xia

Affiliations

Soon will be listed here.

Abstract

MicroRNA-10a (miR-10a) has a wide range of functions in nearly all mammalian tissues and is involved in the occurrence of many diseases. However, it remains unknown whether miR-10a is associated with human recurrent spontaneous abortion (RSA). In this study, we found that rs3809783 A > T in miR-10a coding region was significantly associated with the increase of the risk of human unexplained RSA (URSA) acquisition in a Han-Chinese population. The T allele of rs3809783 hindered the production of mature miR-10a. A to T substitution in miR-10a rs3809783 repressed cell proliferation and migratory capacity. Further investigation discovered that Bcl-2-interacting mediator (Bim) was the functional target of miR-10a and inversely regulated Bim expression. Dual-luciferase assay indicated that A allele in miR-10a rs3809783 could more effectively suppress Bim expression than T allele. In addition, A to T substitution in miR-10a rs3809783 attenuated the sensibility of cells to progesterone and its antagonist mifepristone. Collectively, our data suggest that rs3809783 A > T in pri-miR-10a may be conductive to the genetic predisposition to RSA by disrupting the production of mature miR-10a and reinforcing the expression of Bim.

Citing Articles

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