Demonstration of GABAergic Cell Bodies in the Suprachiasmatic Nucleus: in Situ Hybridization of Glutamic Acid Decarboxylase (GAD) MRNA and Immunocytochemistry of GAD and GABA

Overview

Journal Neurosci Lett

Specialty Neurology

Date 1989 Jul 31

PMID 2682385

Citations 31

Authors

H Okamura

A Berod

J F Julien

M Geffard

K Kitahama

J Mallet

P Bobillier

Affiliations

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Abstract

The existence of GABAergic neurons in the rat suprachiasmatic nucleus (SCN) was demonstrated by three specific markers; mRNA coding for glutamic acid decarboxylase (GAD) and visualized by in situ hybridization using a 35S-labelled cDNA probe, and GAD protein and GABA were identified by immunocytochemistry using specific antisera. In situ hybridization demonstrated well labelled GAD mRNA positive cells throughout SCN, and GABA and GAD immunoreactive cells showed similar distributions. These results indicate that GABA is a transmitter of a large portion of the SCN neuronal population.

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