Long Non-coding RNA AK027294 Involves in the Process of Proliferation, Migration, and Apoptosis of Colorectal Cancer Cells

Overview

Journal Tumour Biol

Publisher Sage Publications

Specialty Oncology

Date 2016 Jan 29

PMID 26820130

Citations 12

Authors

Hui Niu

Zhaoyang Hu

Hui Liu

Guoliang Hu

Bo Yang

Shixiu Wu

Fang Li

Affiliations

Soon will be listed here.

Abstract

This study is aimed to investigate the differentially expressed long non-coding RNAs (lncRNAs) in colorectal cancer and its potential biological function. Colorectal adenoma is the precancerous lesions of colorectal cancer, so in this study, we used colorectal adenoma as negative control. The global lncRNA expression profile in colorectal cancer and adenoma was evaluated by bioinformatics. The biological functions and potential mechanism of AK027294 were investigated in HCT116, HCT8, and SW480 colorectal cancer cells. A total of 135 lncRNAs were found to be differentially expressed in colorectal cancer and adenoma tissues. Among them, 71 lncRNAs were up-regulated and 64 lncRNAs were down-regulated. Especially, AK027294 was found to be highly expressed in colorectal cancer tissues compared with colorectal adenoma tissues (fold change is 184.5). Our results indicated that AK027294 down-regulation significantly inhibited colorectal cancer cells proliferation and migration, but promoted cell apoptosis (P < 0.05). The potential mechanism of AK027294 might be associated with the regulation of caspase-3, caspase-8, Bcl-2, MMP12, MMP9, and TWIST. The lncRNA expression profile in colorectal cancer suggests lncRNAs may play important roles in the occurrence and progression of colorectal cancer. AK027294 is highly expressed in colorectal cancer and closely correlates with colorectal cells proliferation, migration, and apoptosis.

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References

Valdes-Mora F, Gomez del Pulgar T, Bandres E, Cejas P, Ramirez de Molina A, Perez-Palacios R . TWIST1 overexpression is associated with nodal invasion and male sex in primary colorectal cancer. Ann Surg Oncol. 2008; 16(1):78-87. DOI: 10.1245/s10434-008-0166-x. View

Gao Y, Meng H, Liu S, Hu J, Zhang Y, Jiao T . LncRNA-HOST2 regulates cell biological behaviors in epithelial ovarian cancer through a mechanism involving microRNA let-7b. Hum Mol Genet. 2014; 24(3):841-52. DOI: 10.1093/hmg/ddu502. View

Shi D, Zheng H, Zhuo C, Peng J, Li D, Xu Y . Low expression of novel lncRNA RP11-462C24.1 suggests a biomarker of poor prognosis in colorectal cancer. Med Oncol. 2014; 31(7):31. PMC: 4079943. DOI: 10.1007/s12032-014-0031-7. View

Zheng H, Shi D, Wang Y, Li X, Xu Y, Tripathi P . High expression of lncRNA MALAT1 suggests a biomarker of poor prognosis in colorectal cancer. Int J Clin Exp Pathol. 2014; 7(6):3174-81. PMC: 4097248. View

Guo Q, Zhao Y, Chen J, Hu J, Wang S, Zhang D . BRAF-activated long non-coding RNA contributes to colorectal cancer migration by inducing epithelial-mesenchymal transition. Oncol Lett. 2014; 8(2):869-875. PMC: 4081361. DOI: 10.3892/ol.2014.2154. View

Geisler S, Coller J . RNA in unexpected places: long non-coding RNA functions in diverse cellular contexts. Nat Rev Mol Cell Biol. 2013; 14(11):699-712. PMC: 4852478. DOI: 10.1038/nrm3679. View

Wilusz J, Sunwoo H, Spector D . Long noncoding RNAs: functional surprises from the RNA world. Genes Dev. 2009; 23(13):1494-504. PMC: 3152381. DOI: 10.1101/gad.1800909. View

Pesson M, Volant A, Uguen A, Trillet K, De La Grange P, Aubry M . A gene expression and pre-mRNA splicing signature that marks the adenoma-adenocarcinoma progression in colorectal cancer. PLoS One. 2014; 9(2):e87761. PMC: 3916340. DOI: 10.1371/journal.pone.0087761. View

Iguchi T, Uchi R, Nambara S, Saito T, Komatsu H, Hirata H . A long noncoding RNA, lncRNA-ATB, is involved in the progression and prognosis of colorectal cancer. Anticancer Res. 2015; 35(3):1385-8. View

10.

Kim Y, Kim G, Kwon C, Kim J, Park P, Hahm K . TWIST1 and SNAI1 as markers of poor prognosis in human colorectal cancer are associated with the expression of ALDH1 and TGF-β1. Oncol Rep. 2014; 31(3):1380-8. DOI: 10.3892/or.2014.2970. View

11.

Qi P, Xu M, Ni S, Huang D, Wei P, Tan C . Low expression of LOC285194 is associated with poor prognosis in colorectal cancer. J Transl Med. 2013; 11:122. PMC: 3665590. DOI: 10.1186/1479-5876-11-122. View

12.

Hu Y, Chen H, Yu C, Xu J, Wang J, Qian J . A long non-coding RNA signature to improve prognosis prediction of colorectal cancer. Oncotarget. 2014; 5(8):2230-42. PMC: 4039159. DOI: 10.18632/oncotarget.1895. View

13.

Chung S, Nakagawa H, Uemura M, Piao L, Ashikawa K, Hosono N . Association of a novel long non-coding RNA in 8q24 with prostate cancer susceptibility. Cancer Sci. 2010; 102(1):245-52. DOI: 10.1111/j.1349-7006.2010.01737.x. View

14.

Lee T, Rees C, Blanks R, Moss S, Nickerson C, Wright K . Colonoscopic factors associated with adenoma detection in a national colorectal cancer screening program. Endoscopy. 2014; 46(3):203-11. DOI: 10.1055/s-0033-1358831. View

15.

Buranabunwong N, Ruangrungsi N, Chansriniyom C, Limpanasithikul W . Ethyl acetate extract from Glycosmis parva leaf induces apoptosis and cell-cycle arrest by decreasing expression of COX-2 and altering BCL-2 family gene expression in human colorectal cancer HT-29 cells. Pharm Biol. 2014; 53(4):540-7. DOI: 10.3109/13880209.2014.931442. View

16.

Simpson K, Cawthorne C, Zhou C, Hodgkinson C, Walker M, Trapani F . A caspase-3 'death-switch' in colorectal cancer cells for induced and synchronous tumor apoptosis in vitro and in vivo facilitates the development of minimally invasive cell death biomarkers. Cell Death Dis. 2013; 4:e613. PMC: 3674346. DOI: 10.1038/cddis.2013.137. View

17.

Han J, Rong L, Shi C, Dong X, Wang J, Wang B . Screening of lymph nodes metastasis associated lncRNAs in colorectal cancer patients. World J Gastroenterol. 2014; 20(25):8139-50. PMC: 4081685. DOI: 10.3748/wjg.v20.i25.8139. View

18.

Jin H, Li X, Park M, Kim S . FOXM1-mediated downregulation of uPA and MMP9 by 3,3'-diindolylmethane inhibits migration and invasion of human colorectal cancer cells. Oncol Rep. 2015; 33(6):3171-7. DOI: 10.3892/or.2015.3938. View

19.

Jiang Y, Bikle D . LncRNA profiling reveals new mechanism for VDR protection against skin cancer formation. J Steroid Biochem Mol Biol. 2013; 144 Pt A:87-90. DOI: 10.1016/j.jsbmb.2013.11.018. View

20.

Armaghany T, Wilson J, Chu Q, Mills G . Genetic alterations in colorectal cancer. Gastrointest Cancer Res. 2012; 5(1):19-27. PMC: 3348713. View