Reference Genes for Accessing Differential Expression Among Developmental Stages and Analysis of Differential Expression of OBP Genes in Anastrepha Obliqua

Overview

Journal Sci Rep

Specialty Science

Date 2016 Jan 29

PMID 26818909

Citations 29

Authors

Aline Minali Nakamura

Samira Chahad-Ehlers

Andre Luis A Lima

Cristiane Hayumi Taniguti

Iderval Sobrinho Jr

Felipe Rafael Torres

Reinaldo Alves de Brito

Affiliations

Soon will be listed here.

Abstract

The West Indian fruit fly, Anastrepha obliqua, is an important agricultural pest in the New World. The use of pesticide-free methods to control invasive species such as this reinforces the search for genes potentially useful in their genetic control. Therefore, the study of chemosensory proteins involved with a range of responses to the chemical environment will help not only on the understanding of the species biology but may also help the development of environmentally friendly pest control strategies. Here we analyzed the expression patterns of three OBP genes, Obp19d_2, Obp56a and Obp99c, across different phases of A. obliqua development by qPCR. In order to do so, we tested eight and identified three reference genes for data normalization, rpl17, rpl18 and ef1a, which displayed stability for the conditions here tested. All OBPs showed differential expression on adults and some differential expression among adult stages. Obp99c had an almost exclusive expression in males and Obp56a showed high expression in virgin females. Thereby, our results provide relevant data not only for other gene expression studies in this species, as well as for the search of candidate genes that may help in the development of new pest control strategies.

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References

Shin D, Jin L, Lobo N, Severson D . Transcript profiling of the meiotic drive phenotype in testis of Aedes aegypti using suppressive subtractive hybridization. J Insect Physiol. 2011; 57(9):1220-6. PMC: 3167017. DOI: 10.1016/j.jinsphys.2011.05.014. View

Majerowicz D, Alves-Bezerra M, Logullo R, Fonseca-de-Souza A, Meyer-Fernandes J, Braz G . Looking for reference genes for real-time quantitative PCR experiments in Rhodnius prolixus (Hemiptera: Reduviidae). Insect Mol Biol. 2011; 20(6):713-22. DOI: 10.1111/j.1365-2583.2011.01101.x. View

Lagarde A, Spinelli S, Tegoni M, He X, Field L, Zhou J . The crystal structure of odorant binding protein 7 from Anopheles gambiae exhibits an outstanding adaptability of its binding site. J Mol Biol. 2011; 414(3):401-12. DOI: 10.1016/j.jmb.2011.10.005. View

Schetelig M, Milano A, Saccone G, Handler A . Male only progeny in Anastrepha suspensa by RNAi-induced sex reversion of chromosomal females. Insect Biochem Mol Biol. 2011; 42(1):51-7. DOI: 10.1016/j.ibmb.2011.10.007. View

Tsitsanou K, Thireou T, Drakou C, Koussis K, Keramioti M, Leonidas D . Anopheles gambiae odorant binding protein crystal complex with the synthetic repellent DEET: implications for structure-based design of novel mosquito repellents. Cell Mol Life Sci. 2011; 69(2):283-97. PMC: 11114729. DOI: 10.1007/s00018-011-0745-z. View

Coggins S, Estevez-Lao T, Hillyer J . Increased survivorship following bacterial infection by the mosquito Aedes aegypti as compared to Anopheles gambiae correlates with increased transcriptional induction of antimicrobial peptides. Dev Comp Immunol. 2012; 37(3-4):390-401. DOI: 10.1016/j.dci.2012.01.005. View

Ovesna J, Kucera L, Vaculova K, Strymplova K, Svobodova I, Milella L . Validation of the β-amy1 transcription profiling assay and selection of reference genes suited for a RT-qPCR assay in developing barley caryopsis. PLoS One. 2012; 7(7):e41886. PMC: 3409215. DOI: 10.1371/journal.pone.0041886. View

Chao W, Dogramaci M, Foley M, Horvath D, Anderson J . Selection and validation of endogenous reference genes for qRT-PCR analysis in leafy spurge (Euphorbia esula). PLoS One. 2012; 7(8):e42839. PMC: 3419244. DOI: 10.1371/journal.pone.0042839. View

Rodriguez A, Alquezar B, Pena L . Fruit aromas in mature fleshy fruits as signals of readiness for predation and seed dispersal. New Phytol. 2012; 197(1):36-48. DOI: 10.1111/j.1469-8137.2012.04382.x. View

10.

Leal W . Odorant reception in insects: roles of receptors, binding proteins, and degrading enzymes. Annu Rev Entomol. 2012; 58:373-91. DOI: 10.1146/annurev-ento-120811-153635. View

11.

Cheng D, Zhang Z, He X, Liang G . Validation of reference genes in Solenopsis invicta in different developmental stages, castes and tissues. PLoS One. 2013; 8(2):e57718. PMC: 3585193. DOI: 10.1371/journal.pone.0057718. View

12.

Shi X, Guo W, Wan P, Zhou L, Ren X, Ahmat T . Validation of reference genes for expression analysis by quantitative real-time PCR in Leptinotarsa decemlineata (Say). BMC Res Notes. 2013; 6:93. PMC: 3600673. DOI: 10.1186/1756-0500-6-93. View

13.

Katoh K, Standley D . MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Mol Biol Evol. 2013; 30(4):772-80. PMC: 3603318. DOI: 10.1093/molbev/mst010. View

14.

Jacob F, Guertler R, Naim S, Nixdorf S, Fedier A, Hacker N . Careful selection of reference genes is required for reliable performance of RT-qPCR in human normal and cancer cell lines. PLoS One. 2013; 8(3):e59180. PMC: 3598660. DOI: 10.1371/journal.pone.0059180. View

15.

Zheng W, Peng W, Zhu C, Zhang Q, Saccone G, Zhang H . Identification and expression profile analysis of odorant binding proteins in the oriental fruit fly Bactrocera dorsalis. Int J Mol Sci. 2013; 14(7):14936-49. PMC: 3742281. DOI: 10.3390/ijms140714936. View

16.

Siciliano P, Scolari F, Gomulski L, Falchetto M, Manni M, Gabrieli P . Sniffing out chemosensory genes from the Mediterranean fruit fly, Ceratitis capitata. PLoS One. 2014; 9(1):e85523. PMC: 3885724. DOI: 10.1371/journal.pone.0085523. View

17.

Marsaro Junior A, Deus E, Ronchi-Teles B, Adaime R, Silva Junior R . Species of Anastrepha (Diptera: Tephritidae) captured in a guava orchard (Psidium guajava L., Myrtaceae) in Boa Vista, Roraima, Brazil. Braz J Biol. 2014; 73(4):879-86. DOI: 10.1590/s1519-69842013000400026. View

18.

Yi X, Zhao H, Wang P, Hu M, Zhong G . Bdor\Orco is important for oviposition-deterring behavior induced by both the volatile and non-volatile repellents in Bactrocera dorsalis (Diptera: Tephritidae). J Insect Physiol. 2014; 65:51-6. DOI: 10.1016/j.jinsphys.2014.05.007. View

19.

Wang Y, Tang N, Hui T, Wang S, Zeng X, Li H . Identification of endogenous reference genes for RT-qPCR analysis of plasma microRNAs levels in rats with acetaminophen-induced hepatotoxicity. J Appl Toxicol. 2013; 33(11):1330-6. DOI: 10.1002/jat.2864. View

20.

Livak K, Schmittgen T . Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 2002; 25(4):402-8. DOI: 10.1006/meth.2001.1262. View