Effect of Microgrooves and Fibronectin Conjugation on the Osteoblast Marker Gene Expression and Differentiation

Overview

Journal J Adv Prosthodont

Specialty Dentistry

Date 2016 Jan 28

PMID 26816580

Citations 2

Authors

Su-Jung Park

Richard Leesungbok

Su-Jin Ahn

Byung-Jin Im

Do Yun Lee

Yu-Jin Jee

Joon-Ho Yoon

Taixing Cui

Sang Cheon Lee

Suk Won Lee

Affiliations

Soon will be listed here.

Abstract

Purpose: To determine the effect of fibronectin (FN)-conjugated, microgrooved titanium (Ti) on osteoblast differentiation and gene expression in human bone marrow-derived mesenchymal stem cells (MSCs).

Materials And Methods: Photolithography was used to fabricate the microgrooved Ti, and amine functionalization (silanization) was used to immobilize fibronectin on the titanium surfaces. Osteoblast differentiation and osteoblast marker gene expression were analyzed by means of alkaline phosphatase activity assay, extracellular calcium deposition assay, and quantitative real-time PCR.

Results: The conjugation of fibronectin on Ti significantly increased osteoblast differentiation in MSCs compared with non-conjugated Ti substrates. On the extracellular calcium deposition assays of MSCs at 21 days, an approximately two-fold increase in calcium concentration was observed on the etched 60-µm-wide/10-µm-deep microgrooved surface with fibronectin (E60/10FN) compared with the same surface without fibronectin (E60/10), and a more than four-fold increase in calcium concentration was observed on E60/10FN compared with the non-etched control (NE0) and etched control (E0) surfaces. Through a series of analyses to determine the expression of osteoblast marker genes, a significant increase in all the marker genes except type I collagen α1 mRNA was seen with E60/10FN more than with any of the other groups, as compared with NE0.

Conclusion: The FN-conjugated, microgrooved Ti substrate can provide an effective surface to promote osteoblast differentiation and osteoblast marker gene expression in MSCs.

Citing Articles

Fibronectin and Its Applications in Dentistry and Periodontics: A Cell Behaviour Conditioner.

Shirbhate U, Bajaj P, Pandher J, Durge K Cureus. 2022; 14(10):e30702.

PMID: 36439606 PMC: 9693791. DOI: 10.7759/cureus.30702.

Effect of Fibronectin-Coated Micro-Grooved Titanium Surface on Alignment, Adhesion, and Proliferation of Human Gingival Fibroblasts.

Jian X, Huang W, Wu D, You D, Lin Z, Chen J Med Sci Monit. 2017; 23:4749-4759.

PMID: 28974670 PMC: 5637573. DOI: 10.12659/msm.903883.

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