In Vitro Salivary Pellicles from Adults and Children Have Different Protective Effects Against Erosion

Overview

Journal Clin Oral Investig

Specialty Dentistry

Date 2016 Jan 24

PMID 26800668

Citations 10

Authors

Thiago S Carvalho

Tommy Baumann

Adrian Lussi

Affiliations

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Abstract

Objectives: We aimed at analyzing the protective effects of salivary pellicles, formed with saliva from adults or children, on enamel from permanent or deciduous teeth.

Materials And Methods: Ninety human enamel specimens (45 permanent premolars and 45 deciduous canines) were ground, and the outer 200 μm of enamel was removed. We divided the teeth into three further subgroups: no salivary pellicle (control), adult salivary pellicle (AP), and child salivary pellicle (CP). We collected stimulated saliva from adults and children and placed 160 μl of either saliva on enamel specimens from AP and CP, respectively. Control specimens received no saliva. Specimens were stored at 37 °C for 2 h and then submitted to an erosive challenge (10 mL; 1 % citric acid; pH 3.6; 25 °C, 1 min). Pellicle formation and erosion was repeated for a total of 4 cycles. After every cycle, relative surface reflection intensity (rSRI) and surface microhardness (rSMH) were calculated.

Results: On permanent enamel, AP presented significantly better protective effects, with less rSMH loss (p < 0.001) and less rSRI loss (p < 0.001). On deciduous enamel, CP presented significantly better protective effects than AP and control (p < 0.05), for both measured parameters.

Conclusion: We conclude that pellicles from adults and children promote different erosion protective effects, where adult pellicle provides better protection for permanent enamel, and child pellicle promotes better protection on deciduous enamel.

Clinical Relevance: The present results provide a better understanding toward the protective effect of salivary pellicle against dental erosion and brings light to one more factor involved in the erosion of deciduous teeth.

Citing Articles

Erosion behaviour of human, bovine and equine dental hard tissues.

Hertel S, Basche S, Schmidt V, Staszyk C, Hannig C, Sterzenbach T Sci Rep. 2023; 13(1):19617.

PMID: 37949920 PMC: 10638419. DOI: 10.1038/s41598-023-46759-9.

Protease-inhibitors added to saliva in vitro influence the erosion protective effect of enamel pellicles.

Baumann T, Niemeyer S, Buzalaf M, Carvalho T Sci Rep. 2023; 13(1):8618.

PMID: 37244955 PMC: 10224991. DOI: 10.1038/s41598-023-35334-x.

Differences in susceptibility of deciduous and permanent teeth to erosion exist, albeit depending on protocol design and method of assessment.

Carvalho T, Lussi A, Schlueter N, Baumann T Sci Rep. 2022; 12(1):4153.

PMID: 35264778 PMC: 8907165. DOI: 10.1038/s41598-022-08116-0.

Pellicle Modification with Casein and Mucin Does Not Promote In Vitro Bacterial Biofilm Formation.

Carvalho T, Halter J, Mucolli D, Lussi A, Eick S, Baumann T Oral Health Prev Dent. 2020; 18(3):475-483.

PMID: 32515418 PMC: 11654534. DOI: 10.3290/j.ohpd.a43351.

The use of fluoride for the prevention of dental erosion and erosive tooth wear in children and adolescents.

Lussi A, Buzalaf M, Duangthip D, Anttonen V, Ganss C, Joao-Souza S Eur Arch Paediatr Dent. 2019; 20(6):517-527.

PMID: 30762211 DOI: 10.1007/s40368-019-00420-0.

References

Vukosavljevic D, Custodio W, Buzalaf M, Hara A, Siqueira W . Acquired pellicle as a modulator for dental erosion. Arch Oral Biol. 2014; 59(6):631-8. DOI: 10.1016/j.archoralbio.2014.02.002. View

Anderson P, Hector M, Rampersad M . Critical pH in resting and stimulated whole saliva in groups of children and adults. Int J Paediatr Dent. 2001; 11(4):266-73. DOI: 10.1046/j.1365-263x.2001.00293.x. View

Shellis R, Barbour M, Jesani A, Lussi A . Effects of buffering properties and undissociated acid concentration on dissolution of dental enamel in relation to pH and acid type. Caries Res. 2013; 47(6):601-11. DOI: 10.1159/000351641. View

Carvalho T, Lussi A . Susceptibility of enamel to initial erosion in relation to tooth type, tooth surface and enamel depth. Caries Res. 2015; 49(2):109-15. DOI: 10.1159/000369104. View

Ruhl S, Rayment S, Schmalz G, Hiller K, Troxler R . Proteins in whole saliva during the first year of infancy. J Dent Res. 2004; 84(1):29-34. DOI: 10.1177/154405910508400104. View

Brevik S, Lussi A, Rakhmatullina E . A new optical detection method to assess the erosion inhibition by in vitro salivary pellicle layer. J Dent. 2013; 41(5):428-35. DOI: 10.1016/j.jdent.2013.02.011. View

Schlueter N, Hara A, Shellis R, Ganss C . Methods for the measurement and characterization of erosion in enamel and dentine. Caries Res. 2011; 45 Suppl 1:13-23. DOI: 10.1159/000326819. View

Martins C, Castro G, Siqueira M, Xiao Y, Yamaguti P, Siqueira W . Effect of dialyzed saliva on human enamel demineralization. Caries Res. 2012; 47(1):56-62. DOI: 10.1159/000343574. View

Hannig C, Hannig M, Attin T . Enzymes in the acquired enamel pellicle. Eur J Oral Sci. 2005; 113(1):2-13. DOI: 10.1111/j.1600-0722.2004.00180.x. View

10.

Yao Y, Berg E, Costello C, Troxler R, Oppenheim F . Identification of protein components in human acquired enamel pellicle and whole saliva using novel proteomics approaches. J Biol Chem. 2002; 278(7):5300-8. DOI: 10.1074/jbc.M206333200. View

11.

Cabras T, Pisano E, Boi R, Olianas A, Manconi B, Inzitari R . Age-dependent modifications of the human salivary secretory protein complex. J Proteome Res. 2009; 8(8):4126-34. DOI: 10.1021/pr900212u. View

12.

Siqueira W, Zhang W, Helmerhorst E, Gygi S, Oppenheim F . Identification of protein components in in vivo human acquired enamel pellicle using LC-ESI-MS/MS. J Proteome Res. 2007; 6(6):2152-60. DOI: 10.1021/pr060580k. View

13.

Robinson C, WEATHERELL J, Hallsworth A . Variatoon in composition of dental enamel within thin ground tooth sections. Caries Res. 1971; 5(1):44-57. DOI: 10.1159/000259731. View

14.

Messana I, Cabras T, Iavarone F, Manconi B, Huang L, Martelli C . Chrono-proteomics of human saliva: variations of the salivary proteome during human development. J Proteome Res. 2015; 14(4):1666-77. DOI: 10.1021/pr501270x. View

15.

WEATHERELL J, Robinson C, Hallsworth A . Variations in the chemical composition of human enamel. J Dent Res. 1974; 53(2):180-92. DOI: 10.1177/00220345740530020501. View

16.

Lussi A, Bossen A, Hoschele C, Beyeler B, Megert B, Meier C . Effects of enamel abrasion, salivary pellicle, and measurement angle on the optical assessment of dental erosion. J Biomed Opt. 2012; 17(9):97009-1. DOI: 10.1117/1.JBO.17.9.097009. View

17.

Ben-Aryeh H, Fisher M, Szargel R, Laufer D . Composition of whole unstimulated saliva of healthy children: changes with age. Arch Oral Biol. 1990; 35(11):929-31. DOI: 10.1016/0003-9969(90)90075-l. View

18.

Meurman J, Frank R . Scanning electron microscopic study of the effect of salivary pellicle on enamel erosion. Caries Res. 1991; 25(1):1-6. DOI: 10.1159/000261335. View

19.

Sonju Clasen A, Ruyter I . Quantitative determination of type A and type B carbonate in human deciduous and permanent enamel by means of Fourier transform infrared spectrometry. Adv Dent Res. 1998; 11(4):523-7. DOI: 10.1177/08959374970110042101. View

20.

Zero D, Rahbek I, Fu J, Proskin H, Featherstone J . Comparison of the iodide permeability test, the surface microhardness test, and mineral dissolution of bovine enamel following acid challenge. Caries Res. 1990; 24(3):181-8. DOI: 10.1159/000261263. View