The Activity of Antioxidant Enzymes in Blood Platelets in Different Types of Renal Replacement Therapy: a Cross-sectional Study

Overview

Journal Int Urol Nephrol

Publisher Springer

Specialty Nephrology

Date 2016 Jan 23

PMID 26797857

Citations 6

Authors

Joanna Stepniewska

Barbara Dolegowska

Elzbieta Cecerska-Heryc

Edyta Golembiewska

Alicja Malinowska-Jedraszczyk

Malgorzata Marchelek-Mysliwiec

Kazimierz Ciechanowski

Affiliations

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Abstract

Purpose: The changes in redox status characterise physiological platelet activation. Increased oxidative stress in chronic kidney disease (CKD) associated with uremic toxicity and procedures of renal replacement therapy leads to the impairment of antioxidant properties of platelets. It may contribute to thrombosis and cardiovascular complications increasing morbidity and mortality among the CKD patients. The object of the research was to assess the influence of conservative treatment, peritoneal dialysis and haemodialysis on platelet prooxidative-antioxidative balance.

Methods: The examined group consisted of 122 patients: 37 on regular haemodialysis (HD), 23 on peritoneal dialysis (PD) and 62 on conservative treatment with CKD stages 3-5 (CKD3-5). The activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), glutathione transpherase (GST) in platelets were obtained using kinetic methods. The spectrophotometric method established the concentrations of reduced glutathione (GSH).

Results: SOD activity in PD differs significantly from CKD3-5 (4.96 vs 1.66; p < 0.0001). CAT activity assessed in PD and CKD3-5 was significantly different from HD (0.82 and 0.8 vs 0.52 before and 0.35 after HD, respectively). GST activity reached the highest value in PD (1.62), and it was significantly different from CKD3-5 (0.23) and HD before haemodialysis (0.11). During haemodialysis therapy, there was a considerable increase in GST activity (0.11 vs 0.3; p = 0.02) and decrease in SOD activity (from 3.41 to 2.27; p = 0.01). The highest GSH concentrations were obtained in CKD3-5 and differ significantly from HD (4.12 vs 2.01; p = 0.02).

Conclusions: The type of treatment, age and duration of renal replacement therapy determined significant changes in platelet antioxidative enzymes activities and concentration of GSH, which may enhance the thrombotic complications. PD is associated with lower platelet oxidative stress.

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