Organoid Culture Systems for Prostate Epithelial and Cancer Tissue

Overview

Journal Nat Protoc

Specialties Biology
Pathology
Science

Date 2016 Jan 23

PMID 26797458

Citations 345

Authors

Jarno Drost

Wouter R Karthaus

Dong Gao

Else Driehuis

Charles L Sawyers

Yu Chen

Hans Clevers

Affiliations

Soon will be listed here.

Abstract

This protocol describes a strategy for the generation of 3D prostate organoid cultures from healthy mouse and human prostate cells (either bulk or FACS-sorted single luminal and basal cells), metastatic prostate cancer lesions and circulating tumor cells. Organoids derived from healthy material contain the differentiated luminal and basal cell types, whereas organoids derived from prostate cancer tissue mimic the histology of the tumor. We explain how to establish these cultures in the fully defined serum-free conditioned medium that is required to sustain organoid growth. Starting with the plating of digested tissue material, full-grown organoids can usually be obtained in ∼2 weeks. The culture protocol we describe here is currently the only one that allows the growth of both the luminal and basal prostatic epithelial lineages, as well as the growth of advanced prostate cancers. Organoids established using this protocol can be used to study many different aspects of prostate biology, including homeostasis, tumorigenesis and drug discovery.

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