Parallel Single-cell Sequencing Links Transcriptional and Epigenetic Heterogeneity

Overview

Journal Nat Methods

Specialties Biomedical Engineering
Pathology

Date 2016 Jan 12

PMID 26752769

Citations 370

Authors

Christof Angermueller

Stephen J Clark

Heather J Lee

Iain C Macaulay

Mabel J Teng

Tim Xiaoming Hu

Felix Krueger

Sebastien Smallwood

Chris P Ponting

Thierry Voet

Gavin Kelsey

Oliver Stegle

Wolf Reik

Affiliations

Soon will be listed here.

Abstract

We report scM&T-seq, a method for parallel single-cell genome-wide methylome and transcriptome sequencing that allows for the discovery of associations between transcriptional and epigenetic variation. Profiling of 61 mouse embryonic stem cells confirmed known links between DNA methylation and transcription. Notably, the method revealed previously unrecognized associations between heterogeneously methylated distal regulatory elements and transcription of key pluripotency genes.

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