Effect of Stratification on Surface Properties of Corneal Epithelial Cells

Overview

Journal Invest Ophthalmol Vis Sci

Specialty Ophthalmology

Date 2016 Jan 10

PMID 26747762

Citations 19

Authors

Bernardo Yanez-Soto

Brian C Leonard

Vijay Krishna Raghunathan

Nicholas L Abbott

Christopher J Murphy

Affiliations

Soon will be listed here.

Abstract

Purpose: The purpose of this study was to determine the influence of mucin expression in an immortalized human corneal epithelial cell line (hTCEpi) on the surface properties of cells, such as wettability, contact angle, and surface heterogeneity.

Methods: hTCEpi cells were cultured to confluence in serum-free medium. The medium was then replaced by stratification medium to induce mucin biosynthesis. The mucin expression profile was analyzed using quantitative PCR and Western blotting. Contact angles were measured using a two-immiscible liquid method, and contact angle hysteresis was evaluated by tilting the apparatus and recording advancing and receding contact angles. The spatial distribution of mucins was evaluated with fluorescently labeled lectin.

Results: hTCEpi cells expressed the three main ocular mucins (MUC1, MUC4, and MUC16) with a maximum between days 1 and 3 of the stratification process. Upon stratification, cells caused a very significant increase in contact angle hysteresis, suggesting the development of spatially discrete and heterogeneously distributed surface features, defined by topography and/or chemical functionality. Although atomic force microscopy measurements showed no formation of appreciable topographic features on the surface of the cells, we observed a significant increase in surface chemical heterogeneity.

Conclusions: The surface chemical heterogeneity of the corneal epithelium may influence the dynamic behavior of tear film by "pinning" the contact line between the cellular surface and aqueous tear film. Engineering the surface properties of corneal epithelium could potentially lead to novel treatments in dry eye disease.

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References

Gipson I, Spurr-Michaud S, Argueso P, Tisdale A, Ng T, Russo C . Mucin gene expression in immortalized human corneal-limbal and conjunctival epithelial cell lines. Invest Ophthalmol Vis Sci. 2003; 44(6):2496-506. DOI: 10.1167/iovs.02-0851. View

Raghunathan V, McKee C, Cheung W, Naik R, Nealey P, Russell P . Influence of extracellular matrix proteins and substratum topography on corneal epithelial cell alignment and migration. Tissue Eng Part A. 2013; 19(15-16):1713-22. PMC: 3700063. DOI: 10.1089/ten.TEA.2012.0584. View

Robinson M, Deshpande D, Chou J, Cui W, Smith S, Langefeld C . IL-6 trans-signaling increases expression of airways disease genes in airway smooth muscle. Am J Physiol Lung Cell Mol Physiol. 2015; 309(2):L129-38. PMC: 4504976. DOI: 10.1152/ajplung.00288.2014. View

Lemp M, Holly F, Iwata S, Dohlman C . The precorneal tear film. I. Factors in spreading and maintaining a continuous tear film over the corneal surface. Arch Ophthalmol. 1970; 83(1):89-94. DOI: 10.1001/archopht.1970.00990030091017. View

Livak K, Schmittgen T . Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 2002; 25(4):402-8. DOI: 10.1006/meth.2001.1262. View

Argueso P, Gipson I . Assessing mucin expression and function in human ocular surface epithelia in vivo and in vitro. Methods Mol Biol. 2012; 842:313-25. PMC: 3480083. DOI: 10.1007/978-1-61779-513-8_19. View

DANJO Y, Watanabe H, Tisdale A, George M, Tsumura T, Abelson M . Alteration of mucin in human conjunctival epithelia in dry eye. Invest Ophthalmol Vis Sci. 1998; 39(13):2602-9. View

Yanez-Soto B, Mannis M, Schwab I, Li J, Leonard B, Abbott N . Interfacial phenomena and the ocular surface. Ocul Surf. 2014; 12(3):178-201. DOI: 10.1016/j.jtos.2014.01.004. View

Claes C, WORST J, Zivojnovic R . Retinal detachment surgery following implantation of a keratoprosthesis. A case report. Bull Soc Belge Ophtalmol. 1992; 243:167-9. View

10.

Robertson D, Li L, Fisher S, Pearce V, Shay J, Wright W . Characterization of growth and differentiation in a telomerase-immortalized human corneal epithelial cell line. Invest Ophthalmol Vis Sci. 2005; 46(2):470-8. DOI: 10.1167/iovs.04-0528. View

11.

Watanabe H . Significance of mucin on the ocular surface. Cornea. 2002; 21(2 Suppl 1):S17-22. DOI: 10.1097/00003226-200203001-00005. View

12.

Argueso P, Tisdale A, Spurr-Michaud S, Sumiyoshi M, Gipson I . Mucin characteristics of human corneal-limbal epithelial cells that exclude the rose bengal anionic dye. Invest Ophthalmol Vis Sci. 2005; 47(1):113-9. PMC: 1351157. DOI: 10.1167/iovs.05-0735. View

13.

McKee C, Raghunathan V, Nealey P, Russell P, Murphy C . Topographic modulation of the orientation and shape of cell nuclei and their influence on the measured elastic modulus of epithelial cells. Biophys J. 2011; 101(9):2139-46. PMC: 3207178. DOI: 10.1016/j.bpj.2011.09.042. View

14.

Schaumberg D, Dana R, Buring J, Sullivan D . Prevalence of dry eye disease among US men: estimates from the Physicians' Health Studies. Arch Ophthalmol. 2009; 127(6):763-8. PMC: 2836718. DOI: 10.1001/archophthalmol.2009.103. View

15.

Ferenz K, Waack I, Laudien J, Mayer C, Broecker-Preuss M, de Groot H . Safety of poly (ethylene glycol)-coated perfluorodecalin-filled poly (lactide-co-glycolide) microcapsules following intravenous administration of high amounts in rats. Results Pharma Sci. 2015; 4:8-18. PMC: 4050377. DOI: 10.1016/j.rinphs.2014.04.001. View

16.

Schaumberg D, Sullivan D, Buring J, Dana M . Prevalence of dry eye syndrome among US women. Am J Ophthalmol. 2003; 136(2):318-26. DOI: 10.1016/s0002-9394(03)00218-6. View

17.

Tiffany J . Measurement of wettability of the corneal epithelium. I. Particle attachment method. Acta Ophthalmol (Copenh). 1990; 68(2):175-81. DOI: 10.1111/j.1755-3768.1990.tb01900.x. View

18.

Holly F, Lemp M . Wettability and wetting of corneal epithelium. Exp Eye Res. 1971; 11(2):239-50. DOI: 10.1016/s0014-4835(71)80028-3. View

19.

GOSPODAROWICZ D, Greenburg G, Birdwell C . Determination of cellular shape by the extracellular matrix and its correlation with the control of cellular growth. Cancer Res. 1978; 38(11 Pt 2):4155-71. View

20.

Hori Y, Spurr-Michaud S, Russo C, Argueso P, Gipson I . Differential regulation of membrane-associated mucins in the human ocular surface epithelium. Invest Ophthalmol Vis Sci. 2003; 45(1):114-22. DOI: 10.1167/iovs.03-0903. View