Loss of Y Chromosome in Peripheral Blood of Colorectal and Prostate Cancer Patients

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2016 Jan 9

PMID 26745889

Citations 56

Authors

Predrag Noveski

Svetlana Madjunkova

Emilija Sukarova Stefanovska

Nadica Matevska Geshkovska

Maja Kuzmanovska

Aleksandar Dimovski

Dijana Plaseska-Karanfilska

Affiliations

Soon will be listed here.

Abstract

Background: Although age-related loss of chromosome Y (LOY) in normal hematopoietic cells is a well-known phenomenon, the phenotypic consequences of LOY have been elusive. However, LOY has been found in association with smoking, shorter survival and higher risk of cancer. It was suggested that LOY in blood cells could become a predictive biomarker of male carcinogenesis.

Aims, Methods & Findings: To investigate the association of LOY in blood cells with the risk for development of colorectal (CC) and prostate cancers (PC), we have analyzed DNA samples from peripheral blood of 101 CC male patients (mean age 60.5±11.9 yrs), 70 PC patients (mean age 68.8±8.0 yrs) and 93 healthy control males (mean age 65.8±16.6 yrs). The methodology included co-amplification of homologous sequences on chromosome Y and other chromosomes using multiplex quantitative fluorescent (QF) PCR followed by automatic detection and analysis on ABI 3500 Genetic Analyzer. The mean Y/X ratio was significantly lower in the whole group of cancer patients (0.907±0.12; p = 1.17x10-9) in comparison to the controls (1.015±0.15), as well as in CC (0.884±0.15; p = 3.76x10-9) and PC patients (0.941±0.06; p = 0.00012), when analyzed separately. Multivariate logistic regression analysis adjusting for LOY and age showed that LOY is a more significant predictor of cancer presence than age, and that age probably does not contribute to the increased number of subjects with detectable LOY in cancer patients cohort.

Conclusion: In conclusion, our results support the recent findings of association of LOY in blood cells with carcinogenesis in males.

Citing Articles

The effects of loss of Y chromosome on male health.

Bruhn-Olszewska B, Markljung E, Rychlicka-Buniowska E, Sarkisyan D, Filipowicz N, Dumanski J Nat Rev Genet. 2025; .

PMID: 39743536 DOI: 10.1038/s41576-024-00805-y.

Relationship Between Loss of Y Chromosome and Urologic Cancers: New Future Perspectives.

Russo P, Bizzarri F, Filomena G, Marino F, Iacovelli R, Ciccarese C Cancers (Basel). 2024; 16(22).

PMID: 39594721 PMC: 11593089. DOI: 10.3390/cancers16223766.

The impact of mosaic loss of the Y chromosome (mLOY) in men of advanced age.

Abou Abdallah F, Abdel Massih C, Attieh C, Chebly A Biogerontology. 2024; 25(6):943-955.

PMID: 39223433 DOI: 10.1007/s10522-024-10133-7.

Quantifying Y chromosome loss in primary and metastatic prostate cancer by chromosome painting.

Rajanala S, Ghale R, Nandakumar S, Chadalavada K, Lee G, Stopsack K PLoS One. 2024; 19(4):e0301989.

PMID: 38683764 PMC: 11057730. DOI: 10.1371/journal.pone.0301989.

Loss of the Y Chromosome: A Review of Molecular Mechanisms, Age Inference, and Implications for Men's Health.

Gutierrez-Hurtado I, Sanchez-Mendez A, Becerra-Loaiza D, Rangel-Villalobos H, Torres-Carrillo N, Gallegos-Arreola M Int J Mol Sci. 2024; 25(8).

PMID: 38673816 PMC: 11050192. DOI: 10.3390/ijms25084230.

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