Discovery and Characterization of a Thermostable and Highly Halotolerant GH5 Cellulase from an Icelandic Hot Spring Isolate

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2016 Jan 8

PMID 26741138

Citations 23

Authors

Dimitra Zarafeta

Dimitrios Kissas

Christopher Sayer

Soley R Gudbergsdottir

Efthymios Ladoukakis

Michail N Isupov

Aristotelis Chatziioannou

Xu Peng

Jennifer A Littlechild

Georgios Skretas

Fragiskos N Kolisis

Affiliations

Soon will be listed here.

Abstract

With the ultimate goal of identifying robust cellulases for industrial biocatalytic conversions, we have isolated and characterized a new thermostable and very halotolerant GH5 cellulase. This new enzyme, termed CelDZ1, was identified by bioinformatic analysis from the genome of a polysaccharide-enrichment culture isolate, initiated from material collected from an Icelandic hot spring. Biochemical characterization of CelDZ1 revealed that it is a glycoside hydrolase with optimal activity at 70°C and pH 5.0 that exhibits good thermostability, high halotolerance at near-saturating salt concentrations, and resistance towards metal ions and other denaturing agents. X-ray crystallography of the new enzyme showed that CelDZ1 is the first reported cellulase structure that lacks the defined sugar-binding 2 subsite and revealed structural features which provide potential explanations of its biochemical characteristics.

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