Changes of Testicular Phosphorylated Proteins in Response to Restraint Stress in Male Rats

Overview

Journal J Zhejiang Univ Sci B

Specialties Biology
General Medicine

Date 2016 Jan 8

PMID 26739523

Citations 10

Authors

Supatcharee Arun

Jaturon Burawat

Wannisa Sukhorum

Apichakan Sampannang

Nongnut Uabundit

Sitthichai Iamsaard

Affiliations

Soon will be listed here.

Abstract

Objective: To investigate male reproductive parameters via changes of potential testicular protein markers in restraint-stress rats.

Methods: Male Sprague-Dawley rats were divided into two groups (non-immobilized control and restraint-immobilized/stress groups, n=8 each group). The stress animals were immobilized (12 h/d) by a restraint cage for 7 consecutive days. All reproductive parameters, morphology and histology were observed and compared between groups. In addition, the expression of steroidogenic acute regulatory (StAR) and phosphotyrosine proteins (previously localized in Sertoli and late spermatid cells) in testicular lysate was assayed by immuno-Western blotting.

Results: Testosterone level, sperm concentration and sperm head normality of stress rats were significantly decreased while the corticosterone level was increased as compared with the control (P<0.05). Histologically, stress rats showed low sperm mass in epididymal lumen and some atrophy of seminiferous tubules. Although the expression of testicular StAR protein was not significantly different between groups, changed patterns of the 131, 95, and 75 kDa testicular phosphorylated proteins were observed in the stress group compared with the control group. The intensity of a testicular 95-kDa phosphorylated protein was significantly decreased in stress rats.

Conclusions: This study has demonstrated the alteration of testicular phosphorylated protein patterns, associated with adverse male reproductive parameters in stress rats. It could be an explanation of some infertility in stress males.

Citing Articles

Betaine attenuate chronic restraint stress-induced changes in testicular damage and oxidative stress in male mice.

Meng X, Peng L, Xu J, Guo D, Cao W, Xu Y Reprod Biol Endocrinol. 2022; 20(1):80.

PMID: 35597951 PMC: 9123792. DOI: 10.1186/s12958-022-00949-8.

Effects of treadmill exercise on sexual behavior and reproductive parameters in chronically stressed-male rats.

Kelestimur H, Bulmus O, Serhatlioglu I, Ercan Z, Ozer Kaya S, Yardimci A Physiol Res. 2021; 70(5):765-775.

PMID: 34505528 PMC: 8820524. DOI: 10.33549/physiolres.934585.

Chronic stress increases the tyrosine phosphorylation in female reproductive organs: An experimental study.

Bunsueb S, Lapyuneyong N, Tongpan S, Arun S, Iamsaard S Int J Reprod Biomed. 2021; 19(1):87-96.

PMID: 33554006 PMC: 7851478. DOI: 10.18502/ijrm.v19i1.8183.

Protective effect of melatonin against methotrexate-induced testicular damage in the rat model: An experimental study.

Sukhorum W, Welbat J, Krutsri S, Iamsaard Comma S Int J Reprod Biomed. 2020; 18(5):327-338.

PMID: 32637861 PMC: 7306061. DOI: 10.18502/ijrm.v13i5.7153.

MOTILIPERM Ameliorates Immobilization Stress-Induced Testicular Dysfunction via Inhibition of Oxidative Stress and Modulation of the Nrf2/HO-1 Pathway in SD Rats.

Karna K, Soni K, You J, Choi N, Kim H, Kim C Int J Mol Sci. 2020; 21(13).

PMID: 32635386 PMC: 7370033. DOI: 10.3390/ijms21134750.

References

Wyrobek A, Bruce W . Chemical induction of sperm abnormalities in mice. Proc Natl Acad Sci U S A. 1975; 72(11):4425-9. PMC: 388734. DOI: 10.1073/pnas.72.11.4425. View

Zayachkivska O, Gzhegotsky M, Terletska O, Lutsyk D, Yaschenko A, Dzhura O . Influence of Viburnum opulus proanthocyanidins on stress-induced gastrointestinal mucosal damage. J Physiol Pharmacol. 2007; 57 Suppl 5:155-67. View

Zardooz H, Asl S, Gharib Naseri M, Hedayati M . Effect of chronic restraint stress on carbohydrate metabolism in rat. Physiol Behav. 2006; 89(3):373-8. DOI: 10.1016/j.physbeh.2006.06.023. View

Visconti P, Kopf G . Regulation of protein phosphorylation during sperm capacitation. Biol Reprod. 1998; 59(1):1-6. DOI: 10.1095/biolreprod59.1.1. View

Warzecha Z, Dembinski A, Ceranowicz P, Dembinski M, Cieszkowski J, Kownacki P . Role of sensory nerves in gastroprotective effect of anandamide in rats. J Physiol Pharmacol. 2011; 62(2):207-17. View

Priya P, Girish B, Reddy P . Restraint stress exacerbates alcohol-induced reproductive toxicity in male rats. Alcohol. 2014; 48(8):781-6. DOI: 10.1016/j.alcohol.2014.07.014. View

Almeida S, Petenusci S, Anselmo-Franci J . Decreased spermatogenic and androgenic testicular functions in adult rats submitted to immobilization-induced stress from prepuberty. Braz J Med Biol Res. 1999; 31(11):1443-8. DOI: 10.1590/s0100-879x1998001100013. View

Clarke R, Klock S, Geoghegan A, Travassos D . Relationship between psychological stress and semen quality among in-vitro fertilization patients. Hum Reprod. 1999; 14(3):753-8. DOI: 10.1093/humrep/14.3.753. View

Ernst C, Foldenyi M, Angst J . The Zurich Study: XXI. Sexual dysfunctions and disturbances in young adults. Data of a longitudinal epidemiological study. Eur Arch Psychiatry Clin Neurosci. 1993; 243(3-4):179-88. DOI: 10.1007/BF02190725. View

10.

Bhatia N, Jaggi A, Singh N, Anand P, Dhawan R . Adaptogenic potential of curcumin in experimental chronic stress and chronic unpredictable stress-induced memory deficits and alterations in functional homeostasis. J Nat Med. 2011; 65(3-4):532-43. DOI: 10.1007/s11418-011-0535-9. View

11.

Kwiecien S, Ptak-Belowska A, Krzysiek-Maczka G, Targosz A, Jasnos K, Magierowski M . Asymmetric dimethylarginine, an endogenous inhibitor of nitric oxide synthase, interacts with gastric oxidative metabolism and enhances stress-induced gastric lesions. J Physiol Pharmacol. 2012; 63(5):515-24. View

12.

Iamsaard S, Arun S, Burawat J, Sukhorum W, Wattanathorn J, Nualkaew S . Phenolic contents and antioxidant capacities of Thai-Makham Pom (Phyllanthus emblica L.) aqueous extracts. J Zhejiang Univ Sci B. 2014; 15(4):405-8. PMC: 3989160. DOI: 10.1631/jzus.B1300284. View

13.

Ullrich A, Schlessinger J . Signal transduction by receptors with tyrosine kinase activity. Cell. 1990; 61(2):203-12. DOI: 10.1016/0092-8674(90)90801-k. View

14.

Weissman B, Sottas C, Holmes M, Zhou P, Iadecola C, Hardy D . Normal responses to restraint stress in mice lacking the gene for neuronal nitric oxide synthase. J Androl. 2009; 30(5):614-20. PMC: 2771686. DOI: 10.2164/jandrol.108.007443. View

15.

Xu M, Wei Q, Zheng K, Mao D, Zheng Y, Li Y . Protective effects of Big-leaf mulberry and physiological roles of nitric oxide synthases in the testis of mice following water immersion and restraint stress. Acta Histochem. 2014; 116(8):1323-30. DOI: 10.1016/j.acthis.2014.08.003. View

16.

Zhang M, Shi Z, Yu J, Zhang Y, Wang L, Qiu Y . Scrotal heat stress causes sperm chromatin damage and cysteinyl aspartate-spicific proteinases 3 changes in fertile men. J Assist Reprod Genet. 2015; 32(5):747-55. PMC: 4429437. DOI: 10.1007/s10815-015-0451-0. View

17.

Chotiwat C, Harris R . Antagonism of specific corticotropin-releasing factor receptor subtypes selectively modifies weight loss in restrained rats. Am J Physiol Regul Integr Comp Physiol. 2008; 295(6):R1762-73. PMC: 2685294. DOI: 10.1152/ajpregu.00196.2008. View

18.

ORR T, Mann D . Effects of restraint stress on plasma LH and testosterone concentrations, Leydig cell LH/hCG receptors, and in vitro testicular steroidogenesis in adult rats. Horm Behav. 1990; 24(3):324-41. DOI: 10.1016/0018-506x(90)90013-n. View

19.

Priya P, Reddy P . Effect of restraint stress on lead-induced male reproductive toxicity in rats. J Exp Zool A Ecol Genet Physiol. 2012; 317(7):455-65. DOI: 10.1002/jez.1738. View

20.

Lin H, Yuan K, Zhou H, Bu T, Su H, Liu S . Time-course changes of steroidogenic gene expression and steroidogenesis of rat Leydig cells after acute immobilization stress. Int J Mol Sci. 2014; 15(11):21028-44. PMC: 4264210. DOI: 10.3390/ijms151121028. View