How Type 1 Fimbriae Help Escherichia Coli to Evade Extracellular Antibiotics

Overview

Journal Sci Rep

Specialty Science

Date 2016 Jan 6

PMID 26728082

Citations 31

Authors

Ima Avalos Vizcarra

Vahid Hosseini

Philip Kollmannsberger

Stefanie Meier

Stefan S Weber

Markus Arnoldini

Martin Ackermann

Viola Vogel

Affiliations

Soon will be listed here.

Abstract

To survive antibiotics, bacteria use two different strategies: counteracting antibiotic effects by expression of resistance genes or evading their effects e.g. by persisting inside host cells. Since bacterial adhesins provide access to the shielded, intracellular niche and the adhesin type 1 fimbriae increases bacterial survival chances inside macrophages, we asked if fimbriae also influenced survival by antibiotic evasion. Combined gentamicin survival assays, flow cytometry, single cell microscopy and kinetic modeling of dose response curves showed that type 1 fimbriae increased the adhesion and internalization by macrophages. This was caused by strongly decreased off-rates and affected the number of intracellular bacteria but not the macrophage viability and morphology. Fimbriae thus promote antibiotic evasion which is particularly relevant in the context of chronic infections.

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