Nucleotide Sequence of a CDNA Coding for the NADPH-protochlorophyllide Oxidoreductase (PCR) of Barley (Hordeum Vulgare L.) and Its Expression in Escherichia Coli

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1989 Jun 1

PMID 2671659

Citations 49

Authors

R Schulz

K Steinmuller

M Klaas

C Forreiter

S Rasmussen

C Hiller

K Apel

Affiliations

Soon will be listed here.

Abstract

The primary structure of the NADPH-protochlorophyllide oxidoreductase of barley has been deduced from the nucleotide sequence of a cloned full-length cDNA. This cDNA hybridizes to a 1.7 kb RNA whose steady-state level in dark-grown seedlings is drastically reduced upon illumination. The predicted amino acid sequence (388 residues in length) includes a transit peptide of 74 amino acids whose end point has been delimited by sequencing the N-terminus of the mature protein. Expression of the cDNA in Escherichia coli leads to the synthesis of an enzymatically active precursor of the NADPH-protochlorophyllide oxidoreductase. Activity of this protein in bacterial lysates is completely dependent on the presence of NADPH and protochlorophyllide and requires light.

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