Mycobacterium Tuberculosis DNA Increases Vitamin D Receptor MRNA Expression and the Production of Nitric Oxide and Cathelicidin in Human Monocytes

Overview

Journal Malays J Med Sci

Specialty General Medicine

Date 2015 Dec 31

PMID 26715892

Citations 1

Authors

Siswanto Siswanto

Lilik Zuhriyah

Kusworini Handono

Loeki Enggar Fitri

Sumarno Reto Prawiro

Affiliations

Soon will be listed here.

Abstract

Background: The innate immune response to tuberculosis infection may involve the increased production of nitric oxide and cathelicidin due to the up-regulated expression of the vitamin D receptor (VDR), though this proposed mechanism remains controversial. The aim of this study was to determine how the exposure of human monocytes to Mycobacterium tuberculosis (M. tuberculosis) DNA affects the production of nitric oxide and cathelicidin, as well as the expression of VDR.

Methods: This study was performed using monocytes obtained from healthy donors. After 24 h incubation, monocytes were stimulated with M. tuberculosis DNA for 18 h to determine the expression of VDR mRNA and the production of nitric oxide and cathelicidin versus non-stimulated cells (the control group).

Results: The expression of VDR mRNA was higher in the monocytes exposed to M. tuberculosis DNA compared to the control group (P = 0.020). Monocytes exposed to M. tuberculosis DNA also showed significantly increased production of nitric oxide and cathelicidin compared to the control group (P = 0.0001; P = 0.028).

Conclusion: The stimulation of human monocytes with M. tuberculosis DNA increases the expression of the VDR mRNA and the production of nitric oxide and cathelicidin.

Citing Articles

25-Hydroxyvitamin D potentializes extracellular cathelicidin release from human PBMC stimulated ex vivo with either bacterial (LPS) or viral (P: IC) mimetics.

Aldekwer S, Goncalves-Mendes N, Bingula R, Martinroche G, Lanchais K, Rouge S J Physiol Biochem. 2022; 78(2):335-342.

PMID: 34985728 PMC: 8727486. DOI: 10.1007/s13105-021-00868-z.

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