Participation of the Lytic Replicon in Bacteriophage P1 Plasmid Maintenance

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1989 Sep 1

PMID 2670895

Citations 4

Authors

M B Yarmolinsky

E B HANSEN

S Jafri

D K Chattoraj

Affiliations

Soon will be listed here.

Abstract

P1 bacteriophage carries at least two replicons: a plasmid replicon and a viral lytic replicon. Since the isolated plasmid replicon can maintain itself stably at the low copy number characteristic of intact P1 prophage, it has been assumed that this replicon is responsible for driving prophage replication. We provide evidence that when replication from the plasmid replicon is prevented, prophage replication continues, albeit at a reduced rate. The residual plasmid replication is due to incomplete repression of the lytic replicon by the c1 immunity repressor. Incomplete repression was particularly evident in lysogens of the thermoinducible P1 c1.100 prophage, whose replication at 32 degrees C remained almost unaffected when use of the plasmid replicon was prevented. Moreover, the average plasmid copy number of P1 in a P1 c1.100 lysogen was elevated with respect to the copy number of P1 c1+. The capacity of the lytic replicon to act as an auxiliary in plasmid maintenance may contribute to the extraordinary stability of P1 plasmid prophage.

Citing Articles

Virus-Host Interaction Gets . PART I: Phage P1 Enhanced Development in an DksA-Deficient Cell.

Cech G, Kloska A, Krause K, Potrykus K, Cashel M, Szalewska-Palasz A Int J Mol Sci. 2021; 22(11).

PMID: 34072628 PMC: 8198154. DOI: 10.3390/ijms22115890.

Genome of bacteriophage P1.

Lobocka M, Rose D, Plunkett 3rd G, Rusin M, Samojedny A, Lehnherr H J Bacteriol. 2004; 186(21):7032-68.

PMID: 15489417 PMC: 523184. DOI: 10.1128/JB.186.21.7032-7068.2004.

Characterization of the primary immunity region of the Escherichia coli linear plasmid prophage N15.

Lobocka M, Svarchevsky A, Rybchin V, Yarmolinsky M J Bacteriol. 1996; 178(10):2902-10.

PMID: 8631680 PMC: 178027. DOI: 10.1128/jb.178.10.2902-2910.1996.

Isolation of high affinity human antibodies directly from large synthetic repertoires.

Griffiths A, Williams S, Hartley O, Tomlinson I, Waterhouse P, Crosby W EMBO J. 1994; 13(14):3245-60.

PMID: 8045255 PMC: 395221. DOI: 10.1002/j.1460-2075.1994.tb06626.x.

References

Austin S, Sternberg N, Yarmolinsky M . Miniplasmids of bacteriophage P1. I. Stringent plasmid replication does not require elements that regulate the lytic cycle. J Mol Biol. 1978; 120(2):297-309. DOI: 10.1016/0022-2836(78)90069-4. View

Cohen G, Sternberg N . Genetic analysis of the lytic replicon of bacteriophage P1. I. Isolation and partial characterization. J Mol Biol. 1989; 207(1):99-109. DOI: 10.1016/0022-2836(89)90443-9. View

Sternberg N, Austin S, Hamilton D, Yarmolinsky M . Analysis of bacteriophage P1 immunity by using lambda-P1 recombinants constructed in vitro. Proc Natl Acad Sci U S A. 1978; 75(11):5594-8. PMC: 393013. DOI: 10.1073/pnas.75.11.5594. View

Sternberg N . A characterization of bacteriophage P1 DNA fragments cloned in a lambda vector. Virology. 1979; 96(1):129-42. DOI: 10.1016/0042-6822(79)90179-x. View

von Meyenburg K, Hansen F, Riise E, Bergmans H, Meijer M, Messer W . Origin of replication, oriC, of the Escherichia coli K12 chromosome: genetic mapping and minichromosome replication. Cold Spring Harb Symp Quant Biol. 1979; 43 Pt 1:121-8. DOI: 10.1101/sqb.1979.043.01.018. View

. A new pleiotropic bacteriophage P1 mutation, bof, affecting c1 repression activity, the expression of plasmid incompatibility and the expression of certain constitutive prophage genes. Mol Gen Genet. 1979; 174(2):189-202. DOI: 10.1007/BF00268355. View

Iida S, Arber W . On the role of IS1 in the formation of hybrids between the bacteriophage P1 and the R plasmid NR1. Mol Gen Genet. 1980; 177(2):261-70. DOI: 10.1007/BF00267437. View

Oeschger M, Oeschger N, Wiprud G, Woods S . High efficiency temperature-sensitive amber suppressor strains of Escherichia coli K12: isolation of strains with suppressor-enhancing mutations. Mol Gen Genet. 1980; 177(4):545-52. DOI: 10.1007/BF00272662. View

Ow D, Ausubel F . Recombinant P4 bacteriophages propagate as viable lytic phages or as autonomous plasmids in Klebsiella pneumoniae. Mol Gen Genet. 1980; 180(1):165-75. DOI: 10.1007/BF00267366. View

10.

Elespuru R, Yarmolinsky M . A colorimetric assay of lysogenic induction designed for screening potential carcinogenic and carcinostatic agents. Environ Mutagen. 1979; 1(1):65-78. DOI: 10.1002/em.2860010113. View

11.

Bornhoeft J, STODOLSKY M . Lytic cycle replicative forms of bacteriophages P1 and P1dl: concatemer forms. Virology. 1981; 112(2):518-28. DOI: 10.1016/0042-6822(81)90299-3. View

12.

Tsutsui H, Matsubara K . Replication control and switch-off function as observed with a mini-F factor plasmid. J Bacteriol. 1981; 147(2):509-16. PMC: 216071. DOI: 10.1128/jb.147.2.509-516.1981. View

13.

Austin S, Ziese M, Sternberg N . A novel role for site-specific recombination in maintenance of bacterial replicons. Cell. 1981; 25(3):729-36. DOI: 10.1016/0092-8674(81)90180-x. View

14.

Sternberg N, Hamilton D . Bacteriophage P1 site-specific recombination. I. Recombination between loxP sites. J Mol Biol. 1981; 150(4):467-86. DOI: 10.1016/0022-2836(81)90375-2. View

15.

Sternberg N, Hamilton D, Hoess R . Bacteriophage P1 site-specific recombination. II. Recombination between loxP and the bacterial chromosome. J Mol Biol. 1981; 150(4):487-507. DOI: 10.1016/0022-2836(81)90376-4. View

16.

Devlin B, Baumstark B, Scott J . Superimmunity: characterization of a new gene in the immunity region of P1. Virology. 1982; 120(2):360-75. DOI: 10.1016/0042-6822(82)90037-x. View

17.

Austin S, HART F, Abeles A, Sternberg N . Genetic and physical map of a P1 miniplasmid. J Bacteriol. 1982; 152(1):63-71. PMC: 221375. DOI: 10.1128/jb.152.1.63-71.1982. View

18.

Sternberg N, Austin S . Isolation and characterization of P1 minireplicons, lambda-P1:5R and lambda-P1:5L. J Bacteriol. 1983; 153(2):800-12. PMC: 221699. DOI: 10.1128/jb.153.2.800-812.1983. View

19.

Mizusawa S, Ward D . A bacteriophage lambda vector for cloning with BamHI and Sau3A. Gene. 1982; 20(3):317-22. DOI: 10.1016/0378-1119(82)90200-1. View

20.

Walker J, Walker Jr D . Coliphage P1 morphogenesis: analysis of mutants by electron microscopy. J Virol. 1983; 45(3):1118-39. PMC: 256520. DOI: 10.1128/JVI.45.3.1118-1139.1983. View