TPX2 Promotes Migration and Invasion of Human Breast Cancer Cells

Overview

Journal Asian Pac J Trop Med

Specialties Infectious Diseases
Tropical Medicine

Date 2015 Dec 27

PMID 26706681

Citations 23

Authors

Yong Yang

Da-Peng Li

Na Shen

Xiao-Cheng Yu

Jie-Bao Li

Qi Song

Jia-Heng Zhang

Affiliations

Soon will be listed here.

Abstract

Objective: To investigate the expression of targeting protein for Xenopus kinesin-like protein 2 (TPX2) in breast cancer tissue and to explore its role in proliferation, migration and invasion of breast cancer cells.

Methods: The mRNA and protein expressions of TPX2 in breast cancer tissue and cell lines were assessed by quantitative RT-PCR and Western blot. The effect of TPX2 with RNA interference on proliferation, invasion and migration of breast cancer cells was observed by MTT and Transwell assays.

Results: Both mRNA and protein expressions of TPX2 were upregulated in breast cancer tissues compared to tumor-adjacent tissue. TPX2 expression was also upregulated in breast cancer cell lines, and the TPX2 interfered by small interfering RNA could inhibit the proliferation, invasion and migration of breast cancer cells by inhibiting matrix metalloproteinase-2 and matrix metalloproteinase-9.

Conclusions: Significantly upregulated TPX2 expression is observed in breast cancer tissue and cells, and contributes to promote the proliferation, migration and invasion of breast cancer cells.

Citing Articles

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Zhang W, Dong J, Wu Y, Liang X, Suo L, Wang L Biochem Genet. 2024; .

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Role of the TPX2/NCOA5 axis in regulating proliferation, migration, invasion and angiogenesis of breast cancer cells.

Wang T, Zhang F, Zhang P Exp Ther Med. 2023; 25(6):304.

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Breast cancer stage prediction: a computational approach guided by transcriptome analysis.

Athira K, Gopakumar G Mol Genet Genomics. 2022; 297(6):1467-1479.

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Xia X, Li Y Transl Cancer Res. 2022; 9(10):6050-6061.

PMID: 35117216 PMC: 8797465. DOI: 10.21037/tcr-20-704.