Stable Expression of Basic Fibroblast Growth Factor in Chloroplasts of Tobacco

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2015 Dec 26

PMID 26703590

Citations 12

Authors

Yun-Peng Wang

Zheng-Yi Wei

Xiao-Fang Zhong

Chun-Jing Lin

Yu-Hong Cai

Jian Ma

Yu-Ying Zhang

Yan-Zhi Liu

Shao-Chen Xing

Affiliations

Soon will be listed here.

Abstract

Basic fibroblast growth factor (bFGF) is a multifunctional factor in acceleration of cell proliferation, differentiation and transference, and therefore widely used in clinical applications. In this study, expression vector pWX-Nt03 harboring a codon-optimized bFGF gene was constructed and introduced into the tobacco chloroplasts by particle bombardment. After four rounds of selection, bFGF was proved to integrate into the chloroplast genome of regenerated plants and two of four transgenic plants were confirmed to be homoplastomic by PCR and Southern hybridization. ELISA assay indicated that bFGF represented approximately 0.1% of total soluble protein in the leaves of transplastomic tobacco plants. This is the first report of bFGF expression via chloroplast transformation in model plant, providing an additional option for the production of chloroplast-produced therapeutic proteins.

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