Characterization and Multiplexing of 21 Microsatellite Markers for the Herb Noccaea Caerulescens (Brassicaceae)

Overview

Journal Appl Plant Sci

Date 2015 Dec 24

PMID 26697274

Citations 2

Authors

Mathilde Mousset

Elodie Flaven

Fabienne Justy

Juliette Pouzadoux

Cecile Gode

Maxime Pauwels

Cedric Gonneau

Affiliations

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Abstract

Premise Of The Study: Multiplexed microsatellite markers were developed for population genetic studies in the pseudometallophyte Noccaea caerulescens (Brassicaceae), a model species to investigate metal tolerance and hyperaccumulation in higher plants.

Methods And Results: Microsatellite loci were isolated through pyrosequencing of an enriched DNA library. Three multiplexes combining four previously published and 17 newly designed markers were developed. The new markers were screened in metallicolous and nonmetallicolous populations from southern France. The total number of alleles per locus ranged from five to 18. The observed heterozygosity per locus and per population ranged from 0 to 0.83, and expected heterozygosity ranged from 0 to 0.89.

Conclusions: The investigated loci showed reasonable to high levels of polymorphism at the regional scale. The multiplex set should be helpful in investigating genetic diversity, population structure, and demographic history in N. caerulescens at various spatial scales.

Citing Articles

Can zinc pollution promote adaptive evolution in plants? Insights from a one-generation selection experiment.

Nowak J, Frerot H, Faure N, Glorieux C, Line C, Pourrut B J Exp Bot. 2018; 69(22):5561-5572.

PMID: 30215761 PMC: 6255711. DOI: 10.1093/jxb/ery327.

Lower selfing rates in metallicolous populations than in non-metallicolous populations of the pseudometallophyte Noccaea caerulescens (Brassicaceae) in Southern France.

Mousset M, David P, Petit C, Pouzadoux J, Hatt C, Flaven E Ann Bot. 2016; 117(3):507-19.

PMID: 26772770 PMC: 4765546. DOI: 10.1093/aob/mcv191.

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