Targeted Analysis Reveals an Important Role of JAK-STAT-SOCS Genes for Milk Production Traits in Australian Dairy Cattle

Overview

Journal Front Genet

Date 2015 Dec 24

PMID 26697059

Citations 14

Authors

Sondur J Arun

Peter C Thomson

Paul A Sheehy

Mehar S Khatkar

Herman W Raadsma

Peter Williamson

Affiliations

Soon will be listed here.

Abstract

The Janus kinase and signal transducer and activator of transcription (JAK-STAT) pathway genes along with suppressors of cytokine signalling (SOCS) family genes play a crucial role in controlling cytokine signals in the mammary gland and thus mammary gland development. Mammary gene expression studies showed differential expression patterns for all the JAK-STAT pathway genes. Gene expression studies using qRT-PCR revealed differential expression of SOCS2, SOCS4, and SOCS5 genes across the lactation cycle in dairy cows. Using genotypes from 1,546 Australian Holstein-Friesian bulls, a statistical model for an association analysis based on SNPs within 500 kb of JAK-STAT pathway genes, and SOCS genes alone was constructed. The analysis suggested that these genes and pathways make a significant contribution to the Australian milk production traits. There were 24 SNPs close to SOCS1, SOCS3, SOCS5, SOCS7, and CISH genes that were significantly associated with Australian Profit Ranking (APR), Australian Selection Index (ASI), and protein yield (PY). This study supports the view that there may be some merit in choosing SNPs around functionally relevant genes for the selection and genetic improvement schemes for dairy production traits.

Citing Articles

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References

Cummins S, Waters S, Evans A, Lonergan P, Butler S . Genetic merit for fertility traits in Holstein cows: III. Hepatic expression of somatotropic axis genes during pregnancy and lactation. J Dairy Sci. 2012; 95(7):3711-21. DOI: 10.3168/jds.2011-4977. View

Alluwaimi A, Cullor J . Cytokines gene expression patterns of bovine milk during middle and late stages of lactation. J Vet Med B Infect Dis Vet Public Health. 2002; 49(2):105-10. DOI: 10.1046/j.1439-0450.2002.00515.x. View

Linossi E, Babon J, Hilton D, Nicholson S . Suppression of cytokine signaling: the SOCS perspective. Cytokine Growth Factor Rev. 2013; 24(3):241-8. PMC: 3816980. DOI: 10.1016/j.cytogfr.2013.03.005. View

Wall E, CRAWFORD H, Ellis S, Dahl G, McFadden T . Mammary response to exogenous prolactin or frequent milking during early lactation in dairy cows. J Dairy Sci. 2006; 89(12):4640-8. DOI: 10.3168/jds.S0022-0302(06)72514-0. View

Khatkar M, Nicholas F, Collins A, Zenger K, Cavanagh J, Barris W . Extent of genome-wide linkage disequilibrium in Australian Holstein-Friesian cattle based on a high-density SNP panel. BMC Genomics. 2008; 9:187. PMC: 2386485. DOI: 10.1186/1471-2164-9-187. View

Widmann P, Reverter A, Weikard R, Suhre K, Hammon H, Albrecht E . Systems biology analysis merging phenotype, metabolomic and genomic data identifies Non-SMC Condensin I Complex, Subunit G (NCAPG) and cellular maintenance processes as major contributors to genetic variability in bovine feed efficiency. PLoS One. 2015; 10(4):e0124574. PMC: 4398489. DOI: 10.1371/journal.pone.0124574. View

Khatkar M, Zenger K, Hobbs M, Hawken R, Cavanagh J, Barris W . A primary assembly of a bovine haplotype block map based on a 15,036-single-nucleotide polymorphism panel genotyped in holstein-friesian cattle. Genetics. 2007; 176(2):763-72. PMC: 1894606. DOI: 10.1534/genetics.106.069369. View

Chen X, Losman J, Rothman P . SOCS proteins, regulators of intracellular signaling. Immunity. 2000; 13(3):287-90. DOI: 10.1016/s1074-7613(00)00028-5. View

Raven L, Cocks B, Goddard M, Pryce J, Hayes B . Genetic variants in mammary development, prolactin signalling and involution pathways explain considerable variation in bovine milk production and milk composition. Genet Sel Evol. 2014; 46:29. PMC: 4036308. DOI: 10.1186/1297-9686-46-29. View

10.

Hennighausen L, Robinson G, Wagner K, Liu X . Developing a mammary gland is a stat affair. J Mammary Gland Biol Neoplasia. 2000; 2(4):365-72. DOI: 10.1023/a:1026347313096. View

11.

Dahl G . Effects of short day photoperiod on prolactin signaling in dry cows: a common mechanism among tissues and environments?. J Anim Sci. 2007; 86(13 Suppl):10-4. DOI: 10.2527/jas.2007-0311. View

12.

Sheehy P, Riley L, Raadsma H, Williamson P, Wynn P . A functional genomics approach to evaluate candidate genes located in a QTL interval for milk production traits on BTA6. Anim Genet. 2009; 40(4):492-8. DOI: 10.1111/j.1365-2052.2009.01862.x. View

13.

Reverter A, Chan E . Combining partial correlation and an information theory approach to the reversed engineering of gene co-expression networks. Bioinformatics. 2008; 24(21):2491-7. DOI: 10.1093/bioinformatics/btn482. View

14.

Wei J, Ramanathan P, Martin I, Moran C, Taylor R, Williamson P . Identification of gene sets and pathways associated with lactation performance in mice. Physiol Genomics. 2013; 45(5):171-81. DOI: 10.1152/physiolgenomics.00139.2011. View

15.

Wall E, Auchtung-Montgomery T, Dahl G, McFadden T . Short communication: Short-day photoperiod during the dry period decreases expression of suppressors of cytokine signaling in mammary gland of dairy cows. J Dairy Sci. 2005; 88(9):3145-8. DOI: 10.3168/jds.S0022-0302(05)72997-0. View

16.

Linossi E, Nicholson S . Kinase inhibition, competitive binding and proteasomal degradation: resolving the molecular function of the suppressor of cytokine signaling (SOCS) proteins. Immunol Rev. 2015; 266(1):123-33. DOI: 10.1111/imr.12305. View

17.

Imada K, Leonard W . The Jak-STAT pathway. Mol Immunol. 2000; 37(1-2):1-11. DOI: 10.1016/s0161-5890(00)00018-3. View

18.

Moser G, Khatkar M, Hayes B, Raadsma H . Accuracy of direct genomic values in Holstein bulls and cows using subsets of SNP markers. Genet Sel Evol. 2010; 42:37. PMC: 2964565. DOI: 10.1186/1297-9686-42-37. View

19.

Yamaji D, Kang K, Robinson G, Hennighausen L . Sequential activation of genetic programs in mouse mammary epithelium during pregnancy depends on STAT5A/B concentration. Nucleic Acids Res. 2013; 41(3):1622-36. PMC: 3561979. DOI: 10.1093/nar/gks1310. View

20.

Moser G, Tier B, Crump R, Khatkar M, Raadsma H . A comparison of five methods to predict genomic breeding values of dairy bulls from genome-wide SNP markers. Genet Sel Evol. 2010; 41:56. PMC: 2814805. DOI: 10.1186/1297-9686-41-56. View