Spontaneous Nonthymic T Cell Lymphomas in Young Wistar Rats

Spontaneous acute lymphomas and related leukemias, occurring in three of 2,974 male Wistar rats used as controls in toxicity studies during the last 14 years (1974-1987), were examined by light and electron microscopy and by using immunohistochemistry. At autopsy, conspicuous hepatosplenomegaly was noted. Morphologically, tumor cells of all three rats were medium-sized lymphocytes with many mitotic figures proliferating mainly in the spleen, liver, and bone marrow. Virus-like particles were not detected. Immunohistochemically, almost all tumor cells were positive for thy-1 antigen but negative for hematopoietic and differentiation markers such as W3/13, W3/25, OX4, OX8, and OX12. The results suggest that the lymphomas in these three rats were derived from T cells.