Preconcentration and Separation of Mixed-Species Samples Near a Nano-Junction in a Convergent Microchannel

Overview

Journal Sensors (Basel)

Publisher MDPI

Specialty Biotechnology

Date 2015 Dec 23

PMID 26690167

Citations 1

Authors

Ping-Hsien Chiu

Chen-Hsun Weng

Ruey-Jen Yang

Affiliations

Soon will be listed here.

Abstract

A fluidic microchip incorporating a convergent microchannel and a Nafion-nanoporous membrane is proposed for the preconcentration and separation of multi-species samples on a single platform. In the device, sample preconcentration is achieved by means of the ion concentration polarization effect induced at the micro/nano interface under the application of an external electric field, while species separation is achieved by exploiting the different electrophoretic mobilities of the sample components. The experimental results show that the device is capable of detecting C-reactive protein (CRP) with an initial concentration as low as 9.50 × 10(-6) mg/L given a sufficient preconcentration time and driving voltage. In addition, it is shown that a mixed-species sample consisting of three negatively-charged components (bovine serum albumin (BSA), tetramethylrhodamine(TAMRA) isothiocyanate-Dextran and fluorescent polymer beads) can be separated and preconcentrated within 20 min given a driving voltage of 100 V across 1 cm microchannel in length. In general, the present results confirm the feasibility of the device for the immunoassay or detection of various multi-species samples under low concentration in the biochemical and biomedical fields. The novel device can therefore improve the detection limit of traditional medical facilities.

Citing Articles

Force fields of charged particles in micro-nanofluidic preconcentration systems.

Gong L, Ouyang W, Li Z, Han J AIP Adv. 2018; 7(12):125020.

PMID: 29308297 PMC: 5739909. DOI: 10.1063/1.5008365.

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